Rabbit anti-Rat CD31 Monoclonal Antibody for Flow Cytometry - Q3SWT0

Core Product Specifications and Parameters

Biological Background: CD31 Function and Localization

• Platelet endothelial cell adhesion molecule (PECAM-1, CD31) is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, characterized by six extracellular Ig-like domains and a cytoplasmic tail with immunoreceptor tyrosine-based inhibitory motifs (ITIMs).
• CD31 functions as a key adhesion molecule required for leukocyte transendothelial migration under most inflammatory conditions, with Tyr-660 playing a critical role in trafficking from the lateral border recycling compartment (LBRC) during TEM.
• It mediates homophilic interactions that contribute to endothelial cell-cell adhesion at cell junctions, and heterophilic interaction with CD177 facilitates neutrophil transendothelial migration.
• CD31 regulates macrophage-mediated phagocytosis: homophilic ligation transmits a detachment signal to prevent ingestion of viable neighboring leukocytes, while apoptotic leukocytes disable this signal, promoting tethering and clearance.
• CD31 also modulates bradykinin receptor BDKRB2 activation and influences bradykinin- and hyperosmotic shock-induced ERK1/2 signaling in endothelial cells, and it has been implicated in susceptibility to atherosclerosis.
• Subcellularly, CD31 is localized at the cell membrane, enriched in membrane rafts, and concentrated at cell junctions, underscoring its role in endothelial barrier function.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation on serine and tyrosine residues, which are critical for its signaling and adhesive functions.

Experimental Guidance and Technical Tips

• This antibody is provided as an conjugate, optimized for direct flow cytometry detection of CD31 on rat cell surfaces without the need for a secondary antibody.
• Consider titrating the antibody to determine the optimal working concentration for your specific cell type and experimental conditions.
• An appropriate isotype control antibody conjugated to the same fluorophore should be used to assess non-specific binding.
• CD31 is expressed on endothelial cells, platelets, and leukocyte subsets; validate expression in your sample system using known positive and negative controls (e.g., rat endothelial cell lines versus fibroblasts).
• For intracellular staining or when working with permeabilized cells, verify that the recombinant immunogen is accessible and that the fluorophore remains stable under the chosen fixation/permeabilization protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a comprehensive portfolio of IVD raw materials, including this Rabbit anti-Rat CD31 monoclonal antibody for flow cytometry. Our sourcing expertise encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material procurement to support your CD31-related research and assay development. Partner with CamelBio to confidently bridge the gap from concept to clinic with reliable, one-stop supply solutions.