Rabbit Anti-PPBP Polyclonal Antibody for WB and ELISA - P02775

Core Product Specifications and Parameters

Biological Background: PPBP Function and Localization

• PPBP (Platelet basic protein), also known as CXCL7, belongs to the C-X-C chemokine family and is generated by proteolytic processing of a precursor. Multiple active peptides arise from this processing, each with distinct functions.
• LA-PF4 (low‑affinity platelet factor 4) stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also induces plasminogen activator formation and secretion in human synovial cells.
• NAP‑2 (neutrophil‑activating peptide 2) and its truncated variants NAP‑2(73), NAP‑2(74), NAP‑2(1‑66), and NAP‑2(1‑63) are chemoattractants and activators for neutrophils, acting via CXCR1 and CXCR2 receptors.
• TC‑1 and TC‑2 are antibacterial proteins released from activated platelet α‑granules, contributing to innate immune defense.
• CTAP‑III (connective tissue‑activating peptide III) is a growth factor and mitogen; its truncated form CTAP‑III(1‑81) is more potent than full‑length CTAP‑III at desensitizing chemokine‑induced neutrophil activation.
• All forms are secreted, consistent with their roles as extracellular signaling and effector molecules.
• Key structural and functional features include the presence of disulfide bonds and cleavage on pairs of basic residues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 59–128 of human PPBP, a region that may be shared among several processed isoforms. Consider that the polyclonal antibody may detect multiple mature forms in Western blot.
• Predicted molecular weight is 13 kDa; however, N‑terminal processing variants may exhibit shifts in apparent molecular weight. Validate band patterns in appropriate human platelet or leukocyte lysates.
• For ELISA, this antibody can be used either as a capture or detection reagent, but optimal pairing with another antibody should be empirically determined.
• Since PPBP is a secreted protein, cell‑conditioned medium or human plasma/serum may be suitable sample types for detection assays.
• Cross‑reactivity with other CXC chemokines cannot be excluded due to sequence homology; confirm specificity in your experimental system.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, covering the full development continuum from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material procurement. In the area of chemokine and cytokine research, this anti‑PPBP antibody adds to our portfolio, supporting your diagnostic and laboratory workflows with reliable supply capabilities.