Rabbit anti-Pig CD8a Monoclonal Antibody for Flow Cytometry - A0A287A9Y5

Core Product Specifications and Parameters

Biological Background: T-cell surface glycoprotein CD8 alpha chain Function and Localization

• CD8a is a key coreceptor involved in adaptive immunity, specifically in T cell-mediated immune responses.
• The protein is a single-pass type I membrane glycoprotein, with its N-terminal immunoglobulin-like domain exposed extracellularly.
• It contains a signal peptide that directs it to the secretory pathway and ultimately to the cell surface.
• CD8a forms disulfide-linked dimers, which are essential for its interaction with major histocompatibility complex (MHC) class I molecules.
• The cytoplasmic tail undergoes palmitoylation, a modification that anchors the protein to lipid rafts and modulates signaling.
• Subcellular localization is exclusively at the cell membrane, consistent with its role in cell surface recognition.
• As a member of the immunoglobulin superfamily, CD8a is characterized by a conserved Ig-like domain.
• This protein is part of the Sus scrofa reference proteome, indicating a high-quality gene model.

Experimental Guidance and Technical Tips

• This -conjugated antibody is designed for direct flow cytometry detection of pig CD8a on live cells.
• Titrate the antibody to determine the optimal dilution for your specific sample type and experimental conditions.
• Include appropriate isotype control antibodies to distinguish specific from non-specific binding.
• The immunogen corresponds to the extracellular domain (aa 25-184), ensuring recognition of surface-expressed CD8a.
• Validate staining in well-characterized pig lymphocyte populations, such as peripheral blood mononuclear cells (PBMCs) or splenocytes.
• For intracellular staining, a permeabilization step may be required; however, this antibody is primarily recommended for surface detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, supporting research and diagnostic developers from concept to clinic. Our portfolio includes high-quality monoclonal and polyclonal antibodies, recombinant antigens, and ancillary reagents for immunology, infectious diseases, and beyond. This anti-pig CD8a antibody reflects our commitment to providing reliable tools for swine immunology, vaccine research, and T cell studies. We offer bulk quantities, custom sourcing, and technical consultation to streamline your development pipeline.