Rabbit anti-Pig CD27 Monoclonal Antibody for Flow Cytometry - L7QHB1

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27, also known as CD27L receptor, T-cell activation antigen CD27, and tumor necrosis factor receptor superfamily member 7, is a transmembrane glycoprotein belonging to the TNFR superfamily.
• It functions as a costimulatory immune-checkpoint receptor expressed on T-cells, NK-cells, and B-cells, and binds to its ligand CD70/CD27L on B-cells to mediate antigen-specific T-cell activation and expansion.
• The CD70-CD27 pathway plays a critical role in T-cell memory formation by activating TRAF2-PTPN6 signaling, which inhibits LCK phosphorylation and promotes transcriptional adaptations.
• CD27 also provides negative feedback to leukocyte differentiation during immune activation, modulating hematopoiesis, and negatively regulates the function of Th2 lymphocytes in adipose tissue.
• The protein localizes to the cell membrane and features a signal peptide, a cysteine-rich extracellular domain with disulfide bonds, a single transmembrane helix, and a cytoplasmic tail.
• Post-translational modifications include glycosylation, which is typical for membrane receptors and may influence ligand binding and stability.

Experimental Guidance and Technical Tips

• The immunogen covers the ectodomain (aa 20-180), suggesting recognition of native cell-surface CD27; consider using this antibody for flow cytometric detection of pig immune cells.
• As the antibody is directly conjugated to , verify appropriate laser and filter settings (e.g., 488 nm excitation, 530/30 nm emission) and titrate for optimal staining index.
• Include appropriate controls such as unstained cells, isotype control, and FC block to reduce non-specific binding when analyzing samples from pig.
• For activation studies, stimulate T-cells with CD70-expressing cells and monitor CD27 expression changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for immune-checkpoint and oncology applications. This anti-pig CD27 antibody facilitates T-cell activation and memory research, and we extend our sourcing capabilities to rare targets and custom development. Whether you need conjugated antibodies for flow cytometry or end-to-end technical support from concept to clinic, CamelBio is your trusted partner.