Rabbit Anti-p70 S6 Kinase 2 Monoclonal Antibody for WB - Q9UBS0

Core Product Specifications and Parameters

Biological Background: p70 S6 Kinase 2 (RPS6KB2) Function and Localization

• Encoded by the RPS6KB2 gene, this kinase is also referred to as p70S6Kβ, S6K2, SRK, or STK14B, and belongs to the ribosomal S6 kinase family.
• Functions as a serine/threonine-protein kinase that specifically phosphorylates ribosomal protein S6, a component of the 40S ribosomal subunit, thereby regulating protein synthesis.
• Acts downstream of mTORC1 signaling and integrates growth factor and nutrient availability cues to promote cell proliferation, growth, and G1 cell cycle progression; an alternative regulatory mechanism involves the kinase MEAK7, which mediates late endosomal/lysosomal recruitment and activation. Related references: PMID:29750193
• The protein localizes to both the cytoplasm and the nucleus, enabling regulation of distinct translational and transcriptional processes.
• Contains essential kinase domains, including ATP-binding and nucleotide-binding pockets, classifying it within the AGC kinase family.
• Identified as a phosphoprotein in proteomic analyses, indicating post-translational regulation through phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1–120 amino acids of human p70 S6 Kinase 2; confirm sequence homology when using cross-reactive species (mouse, rat) in non-human models.
• For Western blotting, a band near 53 kDa is expected; use positive controls such as HEK293 or HeLa cell lysates where S6K2 is expressed.
• In IHC-P, select tissues with known S6K2 abundance (e.g., testis, certain carcinomas) and optimize antigen retrieval protocols.Consider
• When performing IF/ICC, adjust fixation and permeabilization to detect both cytoplasmic and nuclear signals.Consider
• The antibody may be compatible with ELISA; validate pairing with appropriate capture/detection reagents in the target assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this rabbit monoclonal antibody against p70 S6 Kinase 2 as part of a broad IVD raw material portfolio for kinase signaling research. As your one-stop sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and support for rare target custom development to accelerate your assay pipeline from concept to clinic.