Rabbit Anti-ORC3 Polyclonal Antibody for WB, ELISA - Q9UBD5

Core Product Specifications and Parameters

Biological Background: ORC3 Function and Localization

• ORC3 is a subunit of the origin recognition complex (ORC), which is essential for the initiation of eukaryotic DNA replication.
• The ORC binds replication origins in an ATP-dependent manner, although specific origin sequences in humans remain unidentified.
• ORC3 specifically interacts with histone H3 and H4 trimethylation marks (H3K9me3, H3K27me3, H4K20me3), connecting DNA replication to the epigenetic landscape.
• It localizes primarily to the nucleus and associates with chromosomes to facilitate pre-replication complex assembly.
• The protein undergoes alternative splicing, phosphorylation, and ubiquitination (Ubl conjugation), as indicated by proteomics and structural studies.
• Known aliases include LAT, ORC3L, and LATHEO.
• Its molecular weight is approximately 82 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–240 amino acids of human ORC3; this region is conserved across human, mouse, and rat, supporting cross-reactivity.
• For Western blot (WB), nuclear extracts or chromatin-enriched fractions are recommended to enrich the target protein.
• In ELISA, consider pairing with a capture antibody or using recombinant ORC3 protein to validate specificity.
• Since this is a polyclonal antibody, lot-to-lot variability may affect absolute sensitivity; optimize dilution factors for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing this ORC3 polyclonal antibody alongside validated antibody pairs, monoclonal alternatives, and bulk ancillary reagents. Whether you need antibodies for DNA replication studies, epigenetic research, or diagnostic assay development, our one-stop supply chain streamlines your procurement from concept to clinic.