Rabbit Anti-OGFOD1 Polyclonal Antibody for WB, ELISA - Q8N543

Core Product Specifications and Parameters

Biological Background: OGFOD1 Function and Localization

• OGFOD1 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1), also known as TPA1 (termination and polyadenylation 1 homolog) and uS12 prolyl 3-hydroxylase, is a member of the dioxygenase superfamily.
• It catalyzes the 3-hydroxylation of proline-62 in the ribosomal protein uS12 (RPS23), which is part of the small ribosomal subunit.
• This post-translational modification plays a critical role in regulating the efficiency of translation termination.
• OGFOD1 is involved in the formation of stress granules, implicating it in the cellular response to environmental stress.
• The protein localizes to both the cytoplasm and the nucleus, reflecting its dynamic cellular roles.
• As a dioxygenase, OGFOD1 requires iron and vitamin C as cofactors for its enzymatic activity, and its structure includes a characteristic 2-oxoglutarate and iron-dependent oxygenase domain.
• Alternative splicing of the OGFOD1 gene generates multiple transcript variants, which may contribute to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–140 of human OGFOD1, targeting an N-terminal region; consider this when designing epitope-specific assays.
• For Western blot, a band at approximately 63 kDa is expected; validate specificity using appropriate positive (e.g., human cell lines) and negative controls, and verify molecular weight markers.
• In ELISA applications, ensure the coating antigen and detection system are optimized; pairing with matched antibody pairs may improve sensitivity.
• Cross-reactivity has been confirmed with human samples; reactivity with other species should be tested experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized provider offering one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. Beyond this OGFOD1 antibody for translation and stress research, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to support your diagnostic and life science workflows. Reach out to explore how CamelBio can accelerate your project with reliable, high-quality reagents.