Rabbit Anti-MYH7/β-MHC Polyclonal Antibody for WB, IF/ICC, ELISA - P12883

Core Product Specifications and Parameters

Biological Background: MYH7/β-MHC Function and Localization

• MYH7 encodes myosin-7, also known as myosin heavy chain 7, myosin heavy chain slow isoform (MyHC-slow), and myosin heavy chain, cardiac muscle beta isoform (MyHC-beta).
• Myosin-7 is an actin-based motor protein with ATPase activity, essential for the generation of mechanical force in both cardiac and skeletal muscle contraction.
• It forms the backbone of the thick filaments in the sarcomere, the fundamental contractile unit of striated muscle, by self-assembling into regular bipolar structures.
• Subcellularly, myosin-7 localizes to the cytoplasm, myofibrils, and specifically the sarcomere, where it interacts with actin thin filaments to drive muscle shortening.
• At the protein level, MYH7 is detected in both wild-type and variant Gln-403 forms in skeletal muscle, indicating its expression in both cardiac and skeletal tissues.
• Post-translational modifications include methylation and phosphorylation, which may regulate its activity and interactions; it also contains binding sites for actin, ATP, and calmodulin.
• Variants in MYH7 are associated with cardiomyopathy, and the protein is implicated in several muscle-related diseases, as highlighted by disease variant keywords.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to residues 1700-1800 of human MYH7/β-MHC (NP_000248.2). This region may be well-conserved among species, supporting the observed cross-reactivity with mouse and rat.
• For Western blot (WB), consider using a high molecular weight marker and 4-8% SDS-PAGE gels to effectively resolve the large 223kDa protein.
• For immunofluorescence/immunocytochemistry (IF/ICC), expect sarcomeric striated staining in cardiac and skeletal muscle cells. Validate patterns in positive control samples and include appropriate negative controls.
• ELISA performance can be optimized by using direct coating of the immunizing peptide or recombinant MYH7 protein. Always titrate antibody concentration for best signal-to-noise ratio.
• Given cross-reactivity, this antibody can be used in mouse and rat muscle tissue samples, but confirm reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. For muscle biology and cardiomyopathy research, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents, alongside rare target raw-material sourcing. Let our MYH7/β-MHC rabbit polyclonal antibody be your bridge to reliable cardiac and skeletal muscle studies.