Rabbit anti-Mouse/Rat CD54 Monoclonal Antibody for FC - P13597

Core Product Specifications and Parameters

Biological Background: CD54 Function and Localization

• CD54 (ICAM-1, Ly-47) is a cell adhesion molecule that serves as a receptor for the integrins LFA-1 (ITGAL:ITGB2) and MAC-1 (ITGAM:ITGB2), mediating leukocyte adhesion and transendothelial migration.
• CD54 is a single-pass type I membrane protein localized at the cell surface, with an extracellular region containing immunoglobulin-like domains.
• It is expressed at low levels on lymphocytes, macrophages, and endothelial cells, but is strongly induced upon stimulation in various cell types including fibroblasts and epithelial cells.
• Alternative splicing generates multiple isoforms; the protein undergoes N-glycosylation and forms disulfide bonds, contributing to its structure and function.
• CD54 interaction with LFA-1 calibrates T-cell killing strength, while engagement with MAC-1 on neutrophils promotes adhesion to stimulated endothelium.
• In epithelial cells, CD54 promotes cell aggregation through interaction with MUC1.
• Key post-translational modifications include ubiquitin conjugation, and the protein is classified under the immunoglobulin superfamily of cell adhesion molecules.

Experimental Guidance and Technical Tips

• The antibody is generated against a recombinant fusion protein covering amino acids 28-485 of mouse CD54 (NP_034623.1), encompassing the extracellular domain, which is suitable for recognizing native cell surface protein in flow cytometry.
• For FC applications, titrate the antibody in the relevant sample system to determine the optimal concentration.
• Consider using appropriate isotype controls and include dead cell exclusion markers for accurate analysis of leukocyte populations.
• The conjugated fluorophore is compatible with common blue laser (488 nm) equipped flow cytometers; ensure proper compensation when multiplexing with other fluorophores.
• The antibody cross-reacts with both mouse and rat; validate reactivity in the target species and tissue type under experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and institutes a streamlined sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For projects involving immune adhesion, inflammation, or leukocyte trafficking research, CamelBio can help source specialized reagents such as this anti-CD54 monoclonal antibody, supporting your workflow from concept to clinic with expert technical consultation and reliable supply chains.