Rabbit anti-Mouse/Rat CD54 mAb - P13597

Core Product Specifications and Parameters

Biological Background: ICAM-1 Function and Localization

• ICAM-1 (Intercellular adhesion molecule 1), also known as CD54, is a cell surface glycoprotein encoded by the Icam1 gene in mice.
• It serves as a ligand for the leukocyte integrins LFA-1 (ITGAL:ITGB2) and MAC-1 (ITGAM:ITGB2), mediating cell-cell adhesion essential for immune surveillance and inflammatory responses.
• ICAM-1 is a member of the immunoglobulin superfamily and contains multiple Ig-like domains, contributing to its adhesive properties.
• The protein is primarily localized on the cell membrane, with low basal expression on lymphocytes, macrophages, and endothelial cells, but is strongly upregulated on various cell types upon stimulation.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and ubiquitin conjugation, which may regulate its stability and function.
• Functionally, ICAM-1 facilitates leukocyte trans-endothelial migration by promoting endothelial cup assembly, and also participates in epithelial cell aggregation through interaction with MUC1.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fusion protein encompassing the extracellular domain (aa 28-485), is predicted to target surface-exposed epitopes of native CD54.
• For flow cytometry (FC), consider titrating the antibody to determine the optimal staining concentration for your specific cell type and treatment condition.
• As CD54 expression is inducible by inflammatory stimuli, validate basal versus activated expression levels in your experimental system, e.g., using cytokine-treated versus untreated cells.
• When using multiple fluorophores, ensure proper compensation, especially if combining with other PE or APC conjugates; is excitable by standard blue or green-yellow lasers.

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