Rabbit anti-Mouse/Rat CD27 Monoclonal Antibody for Flow Cytometry - P41272

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 is a costimulatory immune-checkpoint receptor, also known as T-cell activation antigen CD27, CD27L receptor, and tumor necrosis factor receptor superfamily member 7 (TNFRSF7). It is expressed on the surface of T-cells, NK-cells, and B-cells.
• CD27 binds to its ligand CD70 (CD27L) to mediate antigen-specific T-cell activation and expansion, providing immune surveillance of B-cells. Related references: PMID:11062504
• Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory.
• Activation of CD27 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation, thereby modulating hematopoiesis. Related references: PMID:15723067
• CD27 negatively regulates the function of Th2 lymphocytes in adipose tissue. Related references: PMID:38386557
• Subcellularly, CD27 is localized to the cell membrane.
• Tissue specificity: CD27 is expressed in thymus and spleen, but not in non-lymphoid tissues; it is also found on myeloid progenitor cells. Related references: PMID:8384562, PMID:15723067
• Structurally, CD27 is a transmembrane glycoprotein with a signal peptide, extracellular cysteine-rich repeats, and a cytoplasmic domain; it contains disulfide bonds and belongs to the TNF receptor family.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 24-182 of mouse CD27, corresponding to the extracellular domain; the resulting antibody is expected to recognize the native cell-surface protein.
• Conjugated to , this antibody is suitable for direct flow cytometry. Use appropriate isotype control and Fc receptor blocking to minimize nonspecific binding.
• The antibody cross-reacts with rat CD27; thus, it can be applied to both mouse and rat models. Validate the staining pattern in your specific cell type and treatment conditions.
• For optimal results, titrate the antibody and ensure single-cell suspensions. Consider using a viability dye to exclude dead cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. The CD27 antibody product supports immunology and immune-oncology research, enabling flow cytometric analysis of T-cell activation and memory. Leverage CamelBio’s expertise to secure rare-target raw materials and accelerate your diagnostic development pipeline.