Rabbit anti-Mouse/Rat CD27 Monoclonal Antibody for FC - P41272

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 (TNFRSF7) is a member of the tumor necrosis factor receptor superfamily, serving as a costimulatory immune-checkpoint receptor.
• Engagement by its ligand CD70 on B-cells triggers T-cell activation and expansion, promoting immune surveillance (PubMed:11062504). Related references: PMID:11062504
• CD70 ligation activates the TRAF2-PTPN6 signaling axis, which inhibits LCK phosphorylation to facilitate T-cell memory formation (By similarity).
• In early myeloid progenitor cells, CD70-CD27 signaling provides negative feedback to modulate hematopoiesis (PubMed:15723067). Related references: PMID:15723067
• CD27 negatively regulates the function of Th2 lymphocytes in adipose tissue (PubMed:38386557). Related references: PMID:38386557
• Subcellular localization: Cell membrane; single-pass type I transmembrane protein.
• Tissue specificity: Expressed in thymus, spleen, and on myeloid progenitor cells; not detected in non-lymphoid tissues (PubMed:8384562). Related references: PMID:8384562
• The mature protein has a predicted molecular weight of ~28 kDa and contains characteristic extracellular cysteine-rich repeats, a transmembrane helix, and a cytoplasmic tail. It is glycosylated and contains disulfide bonds (UniProt: P41272).

Experimental Guidance and Technical Tips

• This antibody recognizes an extracellular epitope (aa 24-182) and is suitable for native cell surface staining in flow cytometry. Titrate the antibody on a positive control cell line or primary cells (e.g., mouse splenocytes) to determine the optimal working concentration.
• Include appropriate viability dyes or fixable live/dead markers to exclude dead cells from analysis.
• Validate staining in the relevant sample system, and consider Fc receptor blocking if non-specific binding is a concern.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material supplier, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-CD27 antibody serves immunology and immune-oncology applications, and we can further provide validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and rare target sourcing. Let our team streamline your procurement and accelerate your assay development.