Rabbit anti-Mouse/Human CD55 Monoclonal Antibody for Flow Cytometry - Q61475

Core Product Specifications and Parameters

Biological Background: CD55/DAF Function and Localization

• Complement decay-accelerating factor (CD55/DAF) is a GPI-anchored membrane glycoprotein that regulates the complement cascade.
• It recognizes C4b and C3b fragments deposited on cell surfaces, interfering with the formation of C3 and C5 convertases (C4b2a and C3bBb) and thereby inhibiting complement amplification.
• CD55 prevents assembly and accelerates decay of the C3/C5 convertases, protecting host cells from autologous complement-mediated damage.
• The protein is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) moiety.
• It is expressed in brain, secretory epithelia, skeletal muscle, liver, testes, thymus, spleen, and lymph node.
• Contains Sushi (CCP/SCR) domains, disulfide bonds, and N-linked glycosylation sites.
• Functions as a key component of innate immunity, contributing to the regulation of the complement pathway.

Experimental Guidance and Technical Tips

• This antibody is generated against a recombinant fusion protein spanning the extracellular domains (aa 35–361) of mouse CD55. It is pre-conjugated to for direct flow cytometry, eliminating the need for a secondary antibody.
• For flow cytometry, titrate the antibody to optimize the signal-to-noise ratio and use appropriate isotype controls. Fixation and permeabilization are generally not required for surface-expressed CD55, but validate in your specific protocol.
• Confirm cross-reactivity with human and mouse cells, and test in relevant sample systems to ensure performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers studying the complement system and innate immunity, CamelBio offers one-stop access to CD55/DAF antibodies and related reagents to support your assay development and manufacturing needs.