Rabbit anti-Mouse/Human CD55 mAb for FC - Q61475

Core Product Specifications and Parameters

Biological Background: CD55/DAF Function and Localization

• Complement decay-accelerating factor (CD55/DAF) is a GPI-anchored membrane protein encoded by the Cd55 gene in mouse, also known as Daf1.
• CD55 recognizes cell-surface deposited C4b and C3b fragments generated during complement activation, interfering with the catalytic conversion of C2 and factor B to active C2a and Bb.
• By binding C4b and C3b, CD55 prevents the assembly and accelerates the decay of C3 convertase (C4b2a) and C5 convertase (C3bBb), thereby inhibiting complement amplification.
• This decay-accelerating activity protects host cells from autologous complement-mediated damage and is essential for innate immunity.
• CD55 is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) moiety.
• Expression has been detected in diverse mouse tissues including brain, secretory epithelia, skeletal muscle, liver, testes, thymus, spleen, and lymph node.
• Structural hallmarks include multiple Sushi (CCP) domains, disulfide bonds, and N-linked glycosylation sites, consistent with its classification as a member of the regulators of complement activation (RCA) family.
• Keywords: Complement pathway, Innate immunity, GPI-anchor, Glycoprotein, Disulfide bond, Membrane.

Experimental Guidance and Technical Tips

• The antibody is designed for flow cytometry (FC) and is directly conjugated to , minimizing the need for secondary detection steps.
• As the immunogen covers the extracellular region (amino acids 35–361) of mouse CD55, the antibody likely recognizes an epitope accessible on live, unfixed cells.
• Titration of the antibody is recommended to determine the optimal concentration for specific cell types and experimental conditions.
• Because CD55 is GPI-anchored, gentle handling during cell preparation may help preserve surface expression.
• Users working with both mouse and human samples can leverage the reported cross-reactivity; however, cross-species reactivity should be validated in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic developers and researchers with streamlined access to IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies for critical targets like complement regulators. Whether you require bulk quantities of anti-CD55 reagents or complementary ancillary materials, our sourcing network supports efficient procurement. For projects demanding rare specificities or custom formulations, CamelBio connects you to flexible supply solutions built on B2B reliability. Partner with us to accelerate your immunology research and assay development.