Rabbit anti-Mouse VISTA PolymAb® for FC - Q9D659

Core Product Specifications and Parameters

Biological Background: VISTA Function and Localization

• V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA), also known as Dies1, PD-1H, or V-set domain-containing immunoregulatory receptor, is encoded by the Vsir gene in mice.
• Acts as an immunoregulatory receptor that inhibits the T-cell response. Related references: PMID:21383057, PMID:24743150, PMID:25267631
• May promote differentiation of embryonic stem cells by inhibiting BMP4 signaling. Related references: PMID:20042595
• May stimulate MMP14-mediated MMP2 activation, based on similarity evidence.
• Localizes to the cell membrane as a single-pass type I membrane protein.
• Expressed in spleen, thymus, bone marrow, lymph node, and T-cells; also detected at lower levels in non-hematopoietic tissues such as heart, brain, lung, kidney, muscle, ovary, and testis.
• Contains one immunoglobulin-like domain, a signal peptide, and a transmembrane helix; classified under keywords: Cell membrane, Disulfide bond, Glycoprotein, Immunoglobulin domain, Membrane, Phosphoprotein, Receptor, Reference proteome, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 33-191), suggesting strong suitability for detecting cell surface VISTA via flow cytometry.
• For flow cytometry applications, consider titrating the antibody to determine the optimal concentration and using appropriate isotype controls.
• Validate staining in relevant mouse tissues or cell lines known to express VISTA, such as spleen, thymus, or bone marrow-derived cells.

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CamelBio provides diagnostic manufacturers and researchers with a one-stop platform for IVD raw materials, including validated antibody products like this anti-mouse VISTA antibody. From bulk monoclonal antibodies to ancillary reagents, we streamline procurement to support your immunology and immuno-oncology assay development. Let CamelBio be your reliable partner in sourcing high-quality raw materials for every stage of IVD development — from concept to clinic.