Rabbit anti-Mouse VISTA PolymAb® for FC - Q9D659

Core Product Specifications and Parameters

Biological Background: VISTA Function and Localization

• VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation), also known as PD-1H, Dies1, or Gi24, is a transmembrane protein encoded by the Vsir gene.
• It functions as an immunoregulatory receptor that inhibits T-cell responses, playing a critical role in immune checkpoint regulation. Related references: PMID:21383057, PMID:24743150, PMID:25267631
• VISTA may also promote embryonic stem cell differentiation by inhibiting BMP4 signaling. Related references: PMID:20042595
• Additionally, it may stimulate MMP14-mediated MMP2 activation (By similarity).
• VISTA is a cell membrane protein, consistent with its role as a receptor, and contains an immunoglobulin domain, a signal peptide, and a transmembrane helix, with post-translational modifications including glycosylation and phosphorylation.
• It is expressed in a wide range of immune cells and tissues, including CD4+ and CD8+ T-cells, dendritic cells, macrophages, neutrophils, and NK cells, as well as in non-hematopoietic organs.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Consider using appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.
• Because VISTA is expressed on various immune cells, validate the staining pattern in relevant samples such as mouse spleen or lymph node cells.
• This antibody was developed using a recombinant fusion protein corresponding to the extracellular domain (amino acids 33-191) of mouse VISTA; verify its performance in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering a wide range of validated antibodies and related reagents to support immune checkpoint research. We provide high-quality monoclonal antibodies, antibody pairs, and ancillary reagents, as well as custom sourcing for rare targets. Enhance your diagnostic development with our comprehensive supply solutions, from concept to clinic.