Rabbit anti-Mouse VCAM-1/CD106 Monoclonal Antibody for FC - P29533

Core Product Specifications and Parameters

Biological Background: VCAM-1 Function and Localization

• Vascular cell adhesion protein 1 (VCAM-1), also known as CD106, is a cell adhesion glycoprotein encoded by the Vcam1 gene in mice. The UniProt entry P29533 reports a molecular weight of approximately 81 kDa, while the provided product specifications note a protein weight of 38 kDa and 81 kDa, likely corresponding to differentially processed or cleaved isoforms; alternative splicing is documented in the keywords.
• VCAM-1 is predominantly expressed on the surface of endothelial cells and acts as a major regulator of leukocyte adhesion to the endothelium through interactions with integrins, playing a critical role in immune surveillance and inflammatory responses.
• During inflammation, VCAM-1 binds ligands on activated endothelial cells to trigger calcium channel activation and the small GTPase RAC1, facilitating leukocyte transendothelial migration.
• In the bone marrow, VCAM-1 serves as a quality-control checkpoint for hematopoietic cell entry, providing a "don't-eat-me" signal in the context of MHC class-I presentation.
• The protein is localized to the cell membrane and can also be secreted, supporting both juxtacrine and paracrine signaling functions.
• VCAM-1 expression is tissue-specific, observed on inflamed vascular endothelium, macrophage-like cells, dendritic cells, and within the bone marrow.
• Post-translational modifications include disulfide bonds, glycosylation, and ubiquitin-like conjugation, which may modulate its stability and adhesive properties. It belongs to the immunoglobulin superfamily and contains multiple Ig-like domains involved in adhesion.

Experimental Guidance and Technical Tips

• This -conjugated rabbit monoclonal antibody is designed for direct flow cytometry (FC) analysis of mouse VCAM-1. Since the immunogen spans amino acids 25–310, which corresponds to a region of the extracellular domain, the antibody likely recognizes an epitope exposed on the cell surface.
• For FC experiments, consider titrating the antibody to determine the optimal concentration for your specific sample type and instrument settings. Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to enable accurate gating.
• Given VCAM-1’s inducible expression on endothelial cells and its presence on leukocyte subsets, validate the staining pattern in the relevant biological context, such as LPS- or cytokine-stimulated versus unstimulated mouse endothelial cells or bone marrow populations.
• Ensure that the sample preparation protocol includes sufficient blocking of Fc receptors to reduce non-specific binding, especially when analyzing myeloid-lineage cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinical application. This Rabbit anti-Mouse VCAM-1/CD106 monoclonal antibody supports inflammation and immune cell trafficking research, an area where robust, validated reagents are critical. Through our integrated supply chain, we offer validated antibody pairs, optimized bulk monoclonal antibodies, ancillary reagents, and custom sourcing for rare targets, helping you streamline assay development and scale-up efficiently.