Rabbit anti-Mouse VCAM-1/CD106 Monoclonal Antibody for FC - P29533

Core Product Specifications and Parameters

Biological Background: VCAM-1/CD106 Function and Localization

• VCAM-1 (Vascular cell adhesion protein 1, CD106) is a cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells, playing a critical role in immune surveillance and inflammation by mediating leukocyte-endothelial cell interactions.
• Through interaction with integrins, VCAM-1 activates calcium channels and the small GTPase RAC1, leading to leukocyte transendothelial migration during inflammatory responses.
• In the bone marrow, VCAM-1 functions as a quality-control checkpoint for hematopoietic cell entry by providing a 'don't-eat-me' signal in the context of MHC class-I presentation.
• The protein is localized to the cell membrane and can also be found in a secreted form; it is expressed on inflamed vascular endothelium, macrophage-like cells, dendritic cells, and within the bone marrow.
• VCAM-1 is a member of the immunoglobulin superfamily, containing multiple immunoglobulin-like domains and disulfide bonds; it undergoes alternative splicing and post-translational modifications including glycosylation and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant protein covering amino acids 25–310 of mouse VCAM-1, which spans a large portion of the extracellular domain; this design supports detection of the native surface protein by flow cytometry.
• For optimal results in FC, titrate the antibody concentration and use appropriate compensation controls when combined with other fluorophores.
• Validate staining specificity using VCAM-1-positive cell lines (e.g., activated endothelial cells) and include isotype controls; consider testing in relevant mouse models of inflammation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering antibody pairs, bulk reagents, and custom sourcing for inflammation and immunology targets. This anti-VCAM-1 monoclonal antibody is part of a growing portfolio enabling researchers and diagnostic manufacturers to accelerate assay development from concept to clinic. Our one-stop bridge streamlines access to validated antibodies and ancillary reagents tailored to your workflow.