Rabbit anti-Mouse T-bet/Tbx21 Monoclonal Antibody for Intracellular Flow Cytometry - Q9JKD8

Core Product Specifications and Parameters

Biological Background: T-bet/Tbx21 Function and Localization

• Lineage-defining transcription factor: Initiates Th1 lineage development from naive Th precursor cells by activating Th1 genetic programs and repressing Th2 and Th17 programs.
  Related references: PMID:10761931, PMID:17923685, PMID:21095589
• Th1 gene activation: Activates transcription of IFN-gamma (IFNG) and CXCR3 by recruiting chromatin remodeling complexes (KDM6B, SWI/SNF, H3K4me2-methyltransferase) and the super elongation complex (SEC) to establish a permissive chromatin state.
  Related references: PMID:27292648
• Th17 lineage inhibition: Blocks Th17 commitment by suppressing RUNX1-mediated transactivation of the Th17 regulator RORC.
  Related references: PMID:21151104
• Th2 cytokine repression: Suppresses production of IL-4, IL-5, and IL-13 via inhibition of transcription factors GATA3 and NFATC2.
  Related references: PMID:15662016, PMID:21690296, PMID:23616576
• Type-I interferon regulation: Protects Th1 cells from aberrant type-I IFN responses in an IFN-gamma-rich environment by repressing type-I IFN transcription factors and IFN-stimulated genes.
  Related references: PMID:28623086
• Antiviral B-cell responses: Promotes antiviral IgG2a isotype switching and regulates a broad antiviral gene expression program during chronic viral infection.
  Related references: PMID:27430722
• Subcellular localization: Nuclear protein, consistent with its role as a transcription factor.
• Tissue specificity: Expressed specifically in T cells, including regulatory T (Treg) cells.
  Related references: PMID:10761931, PMID:11087660, PMID:28607488

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, optimize fixation and permeabilization (e.g., paraformaldehyde followed by saponin or methanol) to ensure antibody access to nuclear T-bet.
• Titrate the antibody to determine the optimal dilution for your specific cell type and staining conditions; typical starting range is 0.5–5 µg per million cells.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to confirm specificity and set gates.
• Validate the assay in your target mouse tissue or cell culture system, as T-bet expression levels vary with activation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions streamlined access to high-quality IVD raw materials, including this -conjugated anti-T-bet monoclonal antibody for intracellular flow cytometry. In addition to validated antibody pairs and optimized clones, we supply bulk ancillary reagents and can source rare or custom targets to support your immunology and T-cell biology projects from discovery through clinical application.