Rabbit anti-Mouse S100A9 Monoclonal Antibody for Flow Cytometry (Intracellular) - P31725

Core Product Specifications and Parameters

Biological Background: S100A9 Function and Localization

• S100A9 (also known as calgranulin-B, MRP14, p14) is a member of the S100 family of calcium-binding proteins, with a molecular weight of approximately 13 kDa.
• S100A9 is a calcium- and zinc-binding protein that plays a prominent role in the regulation of inflammatory processes and immune response. Related references: PMID:15331440 PMID:17767165 PMID:18403730
• Intracellularly, as part of the calprotectin complex with S100A8, it facilitates leukocyte arachidonic acid trafficking and metabolism, modulates the tubulin-dependent cytoskeleton during migration of phagocytes, and activates the neutrophilic NADPH-oxidase. Related references: PMID:15331440
• Extracellularly, S100A9 exerts pro-inflammatory, antimicrobial, oxidant-scavenging, and apoptosis-inducing activities. Related references: PMID:21382888
• It acts as an alarmin, binding to TLR4 and AGER, which activates MAP-kinase and NF-kappa-B signaling pathways, amplifying the pro-inflammatory cascade. Related references: PMID:17767165 PMID:18403730 PMID:19402754
• S100A9 is localized in the cytoplasm, associated with the cytoskeleton, and can be secreted; it also localizes to the cell membrane.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which regulate its diverse functions.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, fix and permeabilize cells according to standard protocols (e.g., using a fixation/permeabilization kit) to allow antibody access to cytoplasmic S100A9.
• Titrate the -conjugated antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate positive controls (e.g., mouse neutrophils or monocytes, which express high levels of S100A9) and negative controls (isotype control or unstimulated cells) in your assay.
• Consider that S100A9 can form complexes with S100A8; this interaction may influence epitope accessibility, so validate if the antibody detects the monomeric or complexed form in your assay.
• This antibody was raised against the full-length mouse S100a9 sequence (amino acids 1-113), so it may cross-react with other species if sequences are highly conserved, but the supplier only validates mouse reactivity. Test in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and essential ancillary reagents, supporting your assay development from concept to clinic. This high-quality -conjugated monoclonal antibody targeting mouse S100A9 exemplifies our commitment to delivering reliable immunoreagents for inflammatory and immune research applications. Whether you need bulk quantities or rare target raw materials, CamelBio streamlines your sourcing with one-stop access and expert support.