Rabbit anti-Mouse RANK/CD265 Monoclonal Antibody for FC - O35305

Core Product Specifications and Parameters

Biological Background: RANK/CD265 Function and Localization

• Receptor for TNFSF11/RANKL; essential for RANKL-mediated osteoclastogenesis. Related references: PMID:20483727, PMID:23478294, PMID:9878548

• Interaction with EEIG1 promotes osteoclastogenesis by facilitating the transcription of NFATC1 and activation of PLCG2. Related references: PMID:23478294

• Involved in the regulation of interactions between T-cells and dendritic cells. Related references: PMID:9367155

• Located at the cell membrane and in membrane rafts.

• Ubiquitous expression with high levels in trabecular bone, thymus, small intestine, lung, brain and kidney; weakly expressed in spleen and bone marrow.

• Belongs to the TNF receptor superfamily; features a signal peptide, extracellular cysteine-rich repeats, a transmembrane helix, and a cytoplasmic domain. Classified as a glycoprotein and phosphoprotein with metal-binding properties.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 31–213), which is exposed on the cell surface, making this antibody suitable for detecting native RANK/CD265 by flow cytometry.
• As the antibody is directly conjugated to , no secondary antibody is required; proceed to acquisition after staining and washing.
• Titrate the antibody over a range (e.g., 0.1–5 µg per million cells) using known positive (activated T cells) and negative controls to establish optimal staining conditions.
• Mouse RANK is expressed on osteoclast precursors, activated T lymphocytes, and dendritic cells; validate in the appropriate sample system for your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting. This product targets RANK/CD265, a key receptor in bone metabolism and immune signaling, supporting studies in osteoporosis, arthritis, and immuno‑oncology. From validated antibody pairs and bulk ancillary reagents to rare‑target sourcing, CamelBio streamlines your procurement from concept to commercialization.