Rabbit anti-Mouse RANK/CD265 mAb for FC - O35305

Core Product Specifications and Parameters

Biological Background: RANK/CD265 Function and Localization

• RANK (receptor activator of nuclear factor-κB), also designated CD265, is a member of the tumor necrosis factor receptor superfamily (TNFRSF11A) and is known as the osteoclast differentiation factor receptor (ODFR).
• It serves as the primary receptor for the cytokine RANKL (TNFSF11/TRANCE/OPGL) and is indispensable for RANKL-driven osteoclastogenesis. Related references: PMID:20483727 PMID:23478294 PMID:9878548
• Interaction with the adaptor EEIG1 enhances RANK signaling, promoting osteoclast differentiation via NFATC1 transcriptional activation and PLCG2 stimulation. Related references: PMID:23478294
• Beyond bone remodeling, RANK participates in the regulation of T-cell–dendritic cell crosstalk. Related references: PMID:9367155
• RANK localizes predominantly to the cell membrane and membrane rafts, consistent with its function as a surface signaling receptor.
• The protein is ubiquitously expressed, with elevated levels in trabecular bone, thymus, small intestine, lung, brain, and kidney; lower expression is observed in spleen and bone marrow.
• Post-translational modifications include glycosylation and phosphorylation; structural features comprise disulfide bonds, a signal peptide, a transmembrane helix, and a metal-binding domain.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular region (aa 31–213), making the antibody suitable for detecting surface RANK/CD265 by flow cytometry without membrane permeabilization.
• As a directly conjugated ABflo® 488 monoclonal antibody, it is ready-to-use for FC; titrate the reagent (e.g., 0.5–5 µg/mL) on a positive control such as mouse osteoclast precursors or dendritic cells to establish the optimal working concentration.
• Include an appropriate isotype control to discriminate specific staining from background.
• Cross-reactivity has been confirmed for mouse; validate performance in your specific mouse tissue or primary cell system.
• For functional or signaling studies, consider using a carrier-free (unlabeled) format to avoid potential interference from the fluorescent tag.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive IVD raw-material portfolio, including this ABflo® 488 monoclonal antibody targeting mouse RANK/CD265 for flow cytometry. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare-target sourcing, we support your diagnostic development pipeline end-to-end. Partner with us for one-stop access to materials and expertise that accelerate your osteoclast biology and immunology research from concept to clinic.