Rabbit anti-Mouse PD-L2/CD273/B7-DC Monoclonal Antibody for Flow Cytometry - Q9WUL5

Core Product Specifications and Parameters

Biological Background: PD-L2/CD273/B7-DC Function and Localization

• PD-L2, also known as B7-DC, butyrophilin B7-DC, or CD273, is encoded by the Pdcd1lg2 gene and belongs to the butyrophilin family of immune regulators.
• It plays a critical role in induction and maintenance of immune tolerance to self.
  Related references: PMID:11224527, PMID:11283156, PMID:12719480
• Acts as a ligand for the inhibitory receptor PDCD1/PD-1, inhibiting T-cell proliferation by blocking cell cycle progression and cytokine production.
  Related references: PMID:11224527, PMID:11283156, PMID:12719480
• PD-L2 is a transmembrane protein localized at the cell membrane.
• It is highly expressed in placenta and liver, with lower expression in heart, spleen, lymph nodes, and thymus; also found on dendritic cells and some tumor cell lines.
• Structural features include an immunoglobulin domain, a transmembrane helix, disulfide bonds, and glycosylation sites.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 20-123), which may help generate antibodies recognizing the native surface protein in flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration and include proper isotype controls.
• Ensure a single-cell suspension and block Fc receptors before staining to minimize non-specific binding.
• The conjugate is compatible with 633-647 nm laser lines; avoid fixatives that may affect fluorophore brightness.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to high-quality IVD raw materials, expert technical consultation, and end-to-end assay development support. Whether advancing immune checkpoint research or developing diagnostic kits targeting PD-L2, our global supply network can source monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents to meet your project needs. Partner with CamelBio to efficiently bridge the gap from concept to clinical application.