Rabbit anti-Mouse Ly-6A/E (Sca-1) Monoclonal Antibody for Flow Cytometry - P08648

Core Product Specifications and Parameters

Biological Background: Integrin alpha-5 Function and Localization

• Integrin alpha-5 (ITGA5, CD49e, VLA-5) is a type I transmembrane protein that forms a heterodimer with integrin beta-1 (ITGB1) to function as a fibronectin receptor, mediating RGD-dependent cell adhesion to fibronectin (FN1), fibrinogen, and fibrillin-1 (FBN1). Related references: PMID:18635536 PMID:25398877 PMID:12807887 PMID:17158881 PMID:33962943

• Binding of PLA2G2A to a distinct site (site 2) on ITGA5:ITGB1 induces conformational changes that enhance ligand binding at the classical site 1, modulating integrin activation. Related references: PMID:18635536 PMID:25398877

• ITGA5:ITGB1 also serves as a receptor for interleukin-1 beta (IL1B), playing an essential role in IL1B signaling. Related references: PMID:29030430

• ITGA5 can pair with integrin beta-3 (ITGB3) to form a receptor for soluble CD40LG, which is required for CD40/CD40LG signaling. Related references: PMID:31331973

• During microbial infection, ITGA5:ITGB1 functions as a receptor for human metapneumovirus, facilitating viral entry.

• In HIV-1 infection, interaction of extracellular viral Tat protein with ITGA5:ITGB1 may promote angiogenesis in Kaposi's sarcoma lesions.

• Subcellularly, ITGA5 localizes to the cell membrane and focal adhesions, which are key sites for cell-matrix adhesion and signaling.

• The protein is expressed in placenta and features multiple structural domains, including calcium-binding and disulfide-bonded repeats, with a molecular weight of approximately 115 kDa.

Experimental Guidance and Technical Tips

• The immunogen used, a recombinant fragment encompassing amino acids 27-111 of mouse Ly-6A/E, corresponds to the extracellular portion of the protein. This region likely presents native epitopes, making the antibody suitable for detecting surface-expressed Sca-1 in flow cytometry.
• As a direct PerCP/Cyanine5.5 conjugate, this antibody eliminates the need for secondary staining, reducing assay time and background. Titrate the antibody to determine optimal staining concentrations for your cell subsets.
• Ly-6A/E (Sca-1) is a GPI-anchored protein commonly used as a marker for mouse hematopoietic stem cells and progenitor cells. Validate the antibody in your specific mouse strain, as expression levels may vary.
• Cross-reactivity is confirmed for mouse; for other species, in silico sequence analysis or empirical testing is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-mouse Ly-6A/E antibody supports flow cytometry applications in stem cell and hematopoietic research. CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD assay development and production.