Rabbit anti-Mouse Ly-6A/E (Sca-1) Monoclonal Antibody for Flow Cytometry - P05533

Core Product Specifications and Parameters

Biological Background: Ly-6A/E (Sca-1) Function and Localization

• Lymphocyte antigen 6A-2/6E-1 (Ly-6A/E), also known as Stem cell antigen 1 (Sca-1) or T-cell-activating protein (TAP), is a mouse cell surface protein encoded by the Ly6a gene.
• It functions in T-cell activation, contributing to signaling pathways involved in immune responses.
• Ly-6A/E is a GPI-anchored glycoprotein located on the external side of the plasma membrane, belonging to the Ly-6/urokinase-type plasminogen activator receptor (uPAR) superfamily.
• The protein contains intramolecular disulfide bonds that stabilize its characteristic three-finger structure.
• It is widely expressed in various tissues, with high expression in hematopoietic stem cells, making it a classic marker for stem cell isolation and characterization.
• Additional post-translational modifications include N-linked glycosylation and a GPI-anchor attachment, which are crucial for membrane localization and function.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , a green-fluorescent dye excited by the 488 nm laser line, enabling one-step staining for flow cytometry without secondary reagents.
• Consider using this antibody for immunophenotyping of mouse hematopoietic stem cells, as Sca-1 is a well-established marker for these populations.
• Validate the antibody in the relevant sample system using appropriate controls (e.g., isotype control, knockout/knockdown cells) to confirm specificity.
• When designing multicolor flow cytometry panels, ensure spectral compatibility with other fluorophores; emits around 520 nm, suitable for standard FITC/GFP channels.
• The immunogen comprises the extracellular domain (amino acids 27–111), targeting the native cell surface form of Ly-6A/E.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio provides high-quality antibodies like this anti-Ly-6A/E (Sca-1) monoclonal antibody for stem cell research and immunology applications. We offer validated monoclonal antibody pairs, optimized bulk reagents, and support for rare target material sourcing to streamline your diagnostic development. Contact us to integrate this product into your assay workflows.