Rabbit anti-Mouse Ly-6A/E (Sca-1) mAb for FC - P08648

Core Product Specifications and Parameters

Biological Background: Integrin alpha-5 Function and Localization

• Integrin alpha-5 (ITGA5, CD49e, VLA-5 alpha chain) pairs with beta-1 to form the fibronectin receptor, recognizing the R-G-D tripeptide sequence in ligands such as fibronectin and fibrinogen. Related references: PMID:18635536, PMID:25398877
• It mediates R-G-D-dependent cell adhesion to fibrillin-1 (FBN1) and fibronectin (FN1), playing a critical role in extracellular matrix interactions. Related references: PMID:12807887, PMID:17158881, PMID:33962943
• Beyond classical ligands, ITGA5:ITGB1 binds IL1B and soluble CD40LG, linking integrin signaling to inflammatory and immune pathways. Related references: PMID:29030430, PMID:31331973
• It localizes to the cell membrane and concentrates at focal adhesions, where it transmits mechanical and biochemical signals.
• The protein undergoes cleavage on basic residues, is heavily glycosylated, contains disulfide bonds, and is phosphorylated, features that regulate its function and stability.
• Tissue expression is reported in placenta, with broader expression inferred from its fundamental role in adhesion and signaling.
• During microbial infection, ITGA5:ITGB1 serves as a receptor for human metapneumovirus, human parvovirus B19 (via ITGA2:ITGB1), and HIV-1 Tat protein, influencing pathogenesis.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 27-111) of mouse Ly-6A/E; for flow cytometry, a typical starting concentration range is 0.1–1 µg per million cells, though titration in the relevant sample system is recommended.
• This antibody is directly conjugated to ; ensure the cytometer configuration includes an appropriate far-red laser (e.g., 633–640 nm excitation) and a 660/20 or equivalent filter.
• Confirm Ly-6A/E expression in your mouse strain, as allelic variations (Ly-6A.2 vs Ly-6E.1) exist; include proper isotype controls and Fc blocking to minimize nonspecific binding.
• For multicolor panels, verify minimal spectral overlap with co-labeled antibodies; compensation controls using single-stained cells or beads are essential.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supports diagnostic developers with a wide portfolio of validated antibodies, including monoclonal and conjugated formats for flow cytometry. This Rabbit anti-Mouse Ly-6A/E (Sca-1) antibody serves stem cell and hematopoietic research, and we offer bulk reagent supply, pair-matched antibodies, and complementary raw materials to accelerate your assay development and clinical translation. From target selection to production-scale delivery, CamelBio bridges your sourcing needs with reliable, research-grade materials.