Rabbit anti-Mouse Ly-49D Monoclonal Antibody for Flow Cytometry - Q60651

Core Product Specifications and Parameters

Biological Background: Ly-49D Function and Localization

• Ly-49D is a member of the killer cell lectin-like receptor family, also designated as Killer cell lectin-like receptor 4, Lymphocyte antigen 49d, or T-cell surface glycoprotein Ly-49D.
• The protein is encoded by the Klra4 gene (aliases: Ly-49d, Ly49-d, Ly49d) in mice.
• Ly-49D functions as a receptor on natural killer (NK) cells, specifically recognizing class I major histocompatibility complex (MHC) molecules.
• It is a cell membrane protein with a signal-anchor topology, ensuring its exposure on the NK cell surface for ligand engagement.
• Structural features include a C-type lectin domain, transmembrane helix, disulfide bonds, and glycosylation sites, consistent with its classification as a glycoprotein.
• The receptor is implicated in NK cell adhesion processes, potentially modulating immune synapse stability.
• Alternative splicing of the Klra4 transcript may generate distinct isoforms, though functional differences remain to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein spanning amino acids 66–263 of mouse Ly-49D, which corresponds to its extracellular lectin-like domain. This design supports recognition of the mature cell-surface form.
• This antibody is directly labeled with for flow cytometry. Consider performing a titration experiment to determine the optimal staining concentration for your specific mouse NK cell population.
• Ly-49D expression can be tested in appropriate mouse strains or NK cell lines (e.g., resting or activated NK cells). Include proper isotype controls and Fc receptor blocking to minimize non-specific binding.
• For multi-color panels, verify that fluorescence does not interfere with other commonly used dyes; careful panel design and compensation are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a single access point for high-quality IVD raw materials. This anti-Ly-49D monoclonal antibody supports natural killer cell receptor studies and can be sourced alongside validated antibody pairs, bulk ancillary reagents, and rare-target raw materials. Whether you need custom conjugations, reproducible lot-to-lot consistency, or end-to-end assay development support, CamelBio streamlines your procurement from concept to clinic.