Rabbit anti-Mouse Ly-49D Monoclonal Antibody for FC - Q60651

Core Product Specifications and Parameters

Biological Background: Ly-49D Function and Localization

• Encodes killer cell lectin-like receptor 4 (KLRA4), also known as lymphocyte antigen 49d (Ly-49D) or T-cell surface glycoprotein Ly-49D, a member of the Ly49 receptor family.
• Functions as an activating receptor on natural killer (NK) cells that recognizes class I MHC molecules, mediating NK cell cytotoxicity and cytokine production.
• Expressed on the cell membrane as a type II transmembrane glycoprotein with a single-pass transmembrane domain and an extracellular C-type lectin domain.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, which are critical for proper folding and ligand binding.
• Alternative splicing generates multiple isoforms that may modulate receptor signaling or ligand specificity.
• Belongs to the killer cell lectin-like receptor family; gene symbol Klra4, located in the natural killer gene complex on mouse chromosome 6.

Experimental Guidance and Technical Tips

• Consider titrating the antibody to determine the optimal concentration for flow cytometry staining of mouse NK cells.
• The immunogen encompasses the extracellular domain (aa 66-263), suggesting reactivity with the membrane-bound form of Ly-49D.
• For best results, validate staining in the relevant mouse sample system, such as splenocytes or purified NK cells, taking into account that receptor expression may vary with activation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to premium IVD raw materials, including validated antibody pairs, monoclonal antibodies, and bulk reagents. The Ly-49D antibody supports immunology research focused on NK cell function and receptor-ligand interactions. Contact us for custom sourcing of rare target antibodies and ancillary reagents tailored to your assay development pipeline.