Rabbit anti-Mouse LAMP1/CD107a Monoclonal Antibody for IF/ICC, FC - P11438

Core Product Specifications and Parameters

Biological Background: LAMP1/CD107a Function and Localization

• LAMP1/CD107a, also known as LGP-120, P2B, or CD107a, is a heavily glycosylated lysosomal membrane glycoprotein with a molecular weight of ~43 kDa.
• Plays a key role in lysosome biogenesis, autophagy, and cholesterol homeostasis, and helps regulate lysosomal pH by inhibiting the proton channel TMEM175 (PubMed:15121881). Related references: PMID:15121881
• In NK cells, LAMP1 is critical for cytotoxicity: it facilitates cytotoxic granule movement, perforin trafficking, and protects NK cells from degranulation-induced self-damage (PubMed:23847195). Related references: PMID:23847195
• Presents carbohydrate ligands to selectins, facilitating cell adhesion (By similarity).
• Implicated in tumor cell metastasis (PubMed:2676155). Related references: PMID:2676155
• Subcellular localization includes lysosomal membrane, late endosomes, endosomes, cell membrane, and cytolytic granule membrane.
• Post-translational modifications include disulfide bonds and extensive glycosylation; the protein contains a signal peptide and a single transmembrane helix.

Experimental Guidance and Technical Tips

• For immunofluorescence (IF/ICC), permeabilization with detergents like Triton X-100 or saponin is recommended to detect intracellular lysosomal LAMP1.
• For flow cytometry, cell surface LAMP1 may be detected on activated NK cells or following degranulation; validate expression in your experimental system.
• Use an appropriate isotype control and consider conjugated secondary antibodies if signal amplification is needed.
• The conjugate is compatible with FITC filter sets; ensure minimal fluorophore quenching during staining and imaging.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting support—from early concept through clinical development. This anti-LAMP1/CD107a antibody is part of our extensive catalog for lysosome biology, autophagy, and cancer research, and we can further assist with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare targets to accelerate your assay development.