Rabbit Anti-Mouse LAMP1/CD107a Monoclonal Antibody for Flow Cytometry - P11438

Core Product Specifications and Parameters

Biological Background: LAMP1/CD107a Function and Localization

• Lysosome-associated membrane glycoprotein 1 (LAMP1), also known as CD107a, LGP-120, or P2B, is a heavily glycosylated type I transmembrane protein and a major component of the lysosomal membrane.
• It is critical for lysosome biogenesis, autophagy, and cholesterol homeostasis (PubMed:15121881). Related references: PMID:15121881
• LAMP1 directly inhibits the proton channel TMEM175 to regulate lysosomal pH, ensuring proper hydrolase activity.
• In natural killer (NK) cells, LAMP1 facilitates cytotoxic granule movement and perforin trafficking, and it protects the cell from autolysis by its own granule contents (PubMed:23847195). Related references: PMID:23847195
• LAMP1 is implicated in tumor cell metastasis (PubMed:2676155). Related references: PMID:2676155
• It localizes to lysosomal, late endosomal, and cell surface membranes, as well as cytolytic granule membranes in immune cells.
• The protein contains N-linked glycosylation sites, disulfide bonds, and a short cytoplasmic tail, characteristic of the LAMP family.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 208–363 of mouse LAMP1, a segment located in the intraluminal domain; consider its accessibility for flow cytometry depending on membrane integrity and permeabilization.
• For detection of surface LAMP1 on live cells (e.g., NK cells), test both permeabilized and non-permeabilized protocols to optimize signal, as the epitope may be less exposed.
• Validate antibody specificity using appropriate knockout or knockdown controls in mouse cell lines.
• Given the reported molecular weight of ~43 kDa, expect a higher apparent mass due to extensive glycosylation; confirm by western blot if multiplexing.
• The antibody is supplied as an conjugate; verify laser/filter compatibility with your flow cytometer (e.g., 405 nm excitation, 450/50 emission).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD-grade antibodies, antigens, and ancillary reagents to support diagnostic developers and research labs. This anti-mouse LAMP1 monoclonal antibody extends our catalog of lysosomal and immune cell markers, and we can supply bulk quantities, custom conjugations, and matched antibody pairs for LAMP1 detection. Contact our team for one-stop sourcing of rare-target materials and technical consultation from concept to clinical application.