Rabbit anti-Mouse Ki67 Monoclonal Antibody for Flow Cytometry (Intracellular) - E9PVX6

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Proliferation marker protein Ki‑67 (antigen KI‑67 homolog), encoded by the Mki67 gene, associates with the surface of mitotic chromosomes and plays a critical role in chromosome dispersion and clustering during mitosis.
• Required to maintain individual mitotic chromosomes dispersed in the cytoplasm after nuclear envelope disassembly, acting as a chromosome repellent during early mitosis and a chromosome attractant during late mitosis. Related references: PMID:27362226
• During early mitosis, Ki‑67 forms extended brush‑like structures covering a large fraction of the chromosome surface; this brush creates a steric and electrostatic charge barrier, functioning as a surfactant to prevent chromosomes from collapsing into a single chromatin mass. Related references: PMID:27362226
• In anaphase, the Ki‑67 brush collapses; dephosphorylation during mitotic exit exposes a conserved basic patch that induces RNA‑dependent liquid‑liquid phase separation, switching Ki‑67 to a chromosome attractant that promotes chromosome clustering.
• Does not contribute to the internal structure of mitotic chromosomes. Related references: PMID:26949251
• Subcellularly localizes to the chromosome, nucleus, and nucleolus; mainly present in proliferating cells. Contains domains for DNA binding (preferring supercoiled and AT‑rich DNA) and is subject to acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen spans the C‑terminal region (aa 1816‑2163) containing the Ki67 repeat domains that are important for chromosome association; this region is relatively conserved, potentially supporting cross‑reactivity in related species.
• Optimized for intracellular flow cytometry; ensure proper fixation and permeabilization protocols to allow antibody access to nuclear Ki67. Include appropriate isotype controls and validate in a known proliferating mouse cell line.
• The large protein size (351kDa) may pose challenges for other techniques such as Western blotting; if exploring other applications, consider extended electrophoresis and transfer times, and always verify performance empirically.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti‑Ki67 antibody supports cell proliferation and cell cycle research. As your sourcing partner, we offer validated antibody pairs, optimized monoclonal antibodies, and rare target raw‑material sourcing to accelerate your assay development.