Rabbit anti-Mouse JAM-1/CD321 Monoclonal Antibody for Flow Cytometry - O88792

Core Product Specifications and Parameters

Biological Background: Junctional adhesion molecule A Function and Localization

• Junctional adhesion molecule A (JAM-A), also known as JAM-1 or CD321, is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, encoded by the F11r gene in mouse.
• JAM-A localizes to tight junctions at cell-cell contacts and is also present on the cell membrane, playing a critical role in epithelial and endothelial barrier function.
• It functions in the early formation of tight junctions by recruiting the polarity protein PARD3, and its association with the PARD6-PARD3 complex may regulate tight junction assembly (PubMed:11447115). Related references: PMID:11447115
• JAM-A is involved in regulating leukocyte transmigration across epithelial barriers, particularly monocyte transmigration, and serves as a ligand for integrin alpha-L/beta-2 in memory T-cell and neutrophil trafficking (PubMed:9660867). Related references: PMID:9660867
• It participates in platelet activation, contributing to thrombosis and hemostasis (By similarity).
• Post-translational modifications include glycosylation and phosphorylation, and the protein contains disulfide bonds and immunoglobulin-like domains essential for its adhesive functions.
• Keywords associated with JAM-A include tight junction, cell adhesion, immune cell trafficking, and membrane signaling.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant immunogen corresponding to the extracellular domain (amino acids 27-242), which may be suitable for detecting surface JAM-A on live cells in flow cytometry.
• For FC, titrate the antibody to determine optimal concentration, and include appropriate isotype controls. Consider fixation/permeabilization for intracellular detection if needed.
• Validate detection in your relevant sample system, as performance may vary across cell types or experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For research on tight junction biology and immune cell trafficking, we offer reliable anti-JAM-1/CD321 antibodies to support your assay development needs. Our comprehensive supply capabilities help streamline your workflow from concept to clinic.