Rabbit anti-Mouse JAM-1/CD321 Monoclonal Antibody for FC - O88792

Core Product Specifications and Parameters

Biological Background: JAM-1/CD321 Function and Localization

• Junctional adhesion molecule A (JAM-A, also known as JAM-1 or CD321) is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• It plays a critical role in tight junction assembly by recruiting the PARD3-PARD6 complex to primordial cell contacts, a process documented in early junction formation (PubMed:11447115). Related references: PMID:11447115
• JAM-1 regulates monocyte transmigration across epithelial barriers, contributing to the maintenance of barrier integrity during inflammation (PubMed:9660867). Related references: PMID:9660867
• It functions as a ligand for integrin αLβ2 (LFA-1), facilitating memory T-cell and neutrophil transmigration (by similarity).
• JAM-1 is also involved in platelet activation (by similarity).
• The protein undergoes post-translational modifications including glycosylation and phosphorylation, and contains an extracellular immunoglobulin domain, a single transmembrane helix, and a cytoplasmic tail.
• Subcellularly, it is primarily located at the cell membrane and concentrated at tight junctions.
• Key biological roles include cell adhesion, tight junction maintenance, leukocyte trafficking, and hemostasis.

Experimental Guidance and Technical Tips

• This antibody was generated against a recombinant fusion protein spanning amino acids 27-242 of mouse JAM-1, which includes the extracellular domain. It is suitable for flow cytometry applications; consider using appropriate isotype controls and optimizing the antibody concentration for your specific cell type.
• For flow cytometry, validate the staining protocol using known JAM-1-expressing cells (e.g., mouse endothelial or epithelial cell lines) and ensure minimal non-specific binding.
• Note that the fluorophore may require compensation in multicolor panels; carefully design your panel to avoid spectral overlap.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents to support your diagnostic development. For tight junction and immunology targets like JAM-1/CD321, we deliver reliable research-use antibodies and can assist with custom procurement of rare raw materials. From concept to clinic, we provide one-stop access to high-quality raw materials and technical consulting to accelerate your assay development.