Rabbit anti-Mouse Integrin alpha 2/CD49b Monoclonal Antibody for Flow Cytometry - Q62469

Core Product Specifications and Parameters

Biological Background: Integrin alpha-2 Function and Localization

• Integrin alpha-2, also known as CD49b, DX5, GPIa, and VLA-2 subunit alpha, is a transmembrane glycoprotein that heterodimerizes with integrin beta-1.
• The alpha-2/beta-1 complex is a primary receptor for collagens, mediating platelet and cell adhesion to collagen-rich matrices.
• Beyond collagen binding, the integrin alpha-2/beta-1 recognizes laminins, collagen C-propeptides, and E-cadherin, thereby influencing diverse cell–matrix and cell–cell interactions.
• Through its receptor function, integrin alpha-2/beta-1 modulates collagen and collagenase gene expression, contributes to force generation, and helps organize newly synthesized extracellular matrix.
• Mice lacking functional integrin alpha-2 die very early in embryogenesis, underscoring its essential developmental role.
• Subcellularly, integrin alpha-2 is localized to the plasma membrane and contains a single-pass transmembrane helix.
• The protein features a large extracellular ligand-binding domain with calcium and magnesium binding sites, multiple disulfide bonds, and characteristic FG-GAP repeats.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 168–366 within the extracellular domain of mouse integrin alpha-2, a region that is likely exposed on live cells, making this antibody conjugate suitable for flow cytometry analysis of surface expression.
• When performing flow cytometry, consider using a live/dead discrimination dye and appropriate isotype controls to distinguish specific staining from background.
• Integrin alpha-2 is expressed on platelets and various other cell types; therefore, appropriate sample preparation (e.g., anticoagulant-drawn blood, gentle tissue dissociation) is critical to maintain surface antigen integrity.
• As this antibody is directly labeled with , it can be combined with other fluorochrome-conjugated antibodies in multicolor panels, but spectral overlap compensation should be carefully validated in your experimental system.
• For species cross-reactivity, mouse samples are the intended target; detection in other species is not guaranteed and would require independent verification.
• Always perform a titration experiment to determine the optimal antibody concentration for your specific cell type and staining protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like integrin alpha-2. Whether you are developing cell adhesion assays or studying extracellular matrix biology, CamelBio’s integrated supply chain and technical support help streamline your workflow from concept to clinic. Contact us to discuss your specific requirements and procurement needs.