Rabbit anti-Mouse Integrin alpha 2/CD49b Monoclonal Antibody for Flow Cytometry - Q62469

Core Product Specifications and Parameters

Biological Background: Integrin alpha-2 Function and Localization

• Integrin alpha-2, also known as CD49b, GPIa, or VLA-2 subunit alpha, is the alpha subunit of the integrin alpha-2/beta-1 heterodimer.
• It functions as a receptor for collagen, laminins, collagen C-propeptides, and E-cadherin, mediating cell adhesion to the extracellular matrix.
• Plays a critical role in platelet adhesion to collagens and in the organization and force generation of newly synthesized extracellular matrix.
• Gene knockout studies in mice result in early embryonic lethality, underscoring its essential role in development.
• The protein is a single-pass type I membrane glycoprotein with a large extracellular domain containing multiple calcium-binding repeats.
• Key structural features include disulfide bonds, glycosylation, and metal-binding sites (calcium and magnesium), which are crucial for ligand binding and integrin activation.
• Contains a signal peptide, a long extracellular region, a transmembrane helix, and a short cytoplasmic tail, typical of integrin alpha subunits.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 168-366) of mouse Integrin alpha-2, which is likely critical for ligand interaction; consider using for blocking or staining under non-denaturing conditions.
• This antibody is validated for flow cytometry (FC); for optimal staining, titrate the antibody using positive control cells (e.g., platelets or activated T cells) that express surface CD49b.
• Since it recognizes mouse Integrin alpha-2 and cross-reacts only with mouse, ensure sample species compatibility; it may not recognize human or rat orthologs.
• For multicolor FC panels, note that the antibody is rabbit monoclonal; use appropriate secondary detection or direct conjugate if needed, and consider isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material partner, CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated monoclonal and polyclonal antibodies, including rare targets like Integrin alpha-2/CD49b. We offer a comprehensive range of ancillary reagents and bulk sourcing solutions, supporting your collagen receptor and cell adhesion research from discovery through assay development. Let CamelBio simplify your procurement with quality-controlled materials tailored to your workflow.