Rabbit anti-Mouse Integrin alpha 2/CD49b Monoclonal Antibody for FC - Q62469

Core Product Specifications and Parameters

Biological Background: Integrin alpha 2 Function and Localization

• Integrin alpha-2 (CD49b, GPIa, DX5) pairs with integrin beta-1 to form the VLA-2 (very late activation antigen-2) receptor, a primary collagen-binding integrin.
• It mediates cell adhesion to collagens, laminins, collagen C-propeptides, and E-cadherin, crucial for platelet adhesion, extracellular matrix organization, and force generation.
• The receptor is involved in modulating collagen and collagenase gene expression, influencing tissue remodeling.
• Mice homozygous for a null mutation in Itga2 die very early in embryogenesis, underscoring its developmental importance.
• It localizes to the cell membrane as a single-pass type I membrane protein with a large extracellular domain containing multiple repeats.
• Keywords: calcium and magnesium binding, disulfide bonds, glycoprotein, integrin family, signal peptide, and transmembrane helix.

Experimental Guidance and Technical Tips

• The conjugate enables direct detection in flow cytometry without a secondary antibody, reducing background and simplifying the staining protocol.
• Since the immunogen covers the extracellular domain (aa 168-366), the antibody likely recognizes an epitope in this region; consider using appropriate isotype controls to distinguish specific binding.
• For mouse samples, validate using mouse integrin alpha-2-expressing cells, such as platelets or fibroblast lines; titration should be performed in the relevant sample system to determine optimal conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical support, streamlining your path from concept to clinic. This -conjugated anti-Integrin alpha 2/CD49b antibody complements our validated monoclonal and polyclonal antibody pairs, bulk ancillary reagents, and custom sourcing for challenging targets. Partner with us for reliable, high-quality reagents to accelerate your immunology, cancer, and platelet biology research and development.