Rabbit anti-Mouse Integrin alpha 1/CD49a Monoclonal Antibody for FC - Q3V3R4

Core Product Specifications and Parameters

Biological Background: Integrin alpha-1 Function and Localization

• Integrin alpha-1, also known as CD49a or VLA-1, is a transmembrane receptor subunit that pairs with integrin beta-1 to form a laminin and collagen receptor.
• The alpha-1/beta-1 integrin specifically recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen, mediating cell-matrix adhesion.
• It is involved in anchorage-dependent negative regulation of EGF-stimulated cell growth, linking extracellular matrix interactions to proliferative signaling.
• Integrin alpha-1 is a membrane glycoprotein featuring a large extracellular domain with repeated sequences, a single-pass transmembrane helix, and a short cytoplasmic tail.
• The protein contains binding sites for divalent cations such as calcium and magnesium, which are critical for integrin structural integrity and ligand-binding activity.
• As a member of the integrin alpha chain family, it plays essential roles in cell adhesion, migration, and signal transduction.
• Post-translational modifications, including disulfide bond formation and glycosylation, are required for proper protein folding and surface expression.
• The receptor is classified under reference proteome status and possesses a signal peptide, indicating its synthesis in the ER and trafficking to the plasma membrane.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (aa 29-1141), which likely preserves native epitopes for surface staining in flow cytometry.
• is a bright far-red dye; ensure adequate compensation, use an appropriate isotype control, and include a viability dye to exclude dead cells.
• Titrate the antibody to determine the optimal concentration for your specific cell type and confirm specificity using integrin alpha-1-positive and -negative controls.
• Standard live-cell surface staining protocols are recommended without permeabilization, unless co-staining for intracellular targets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering a wide range of monoclonal and polyclonal antibodies, antigen-grade proteins, and ancillary reagents to support diagnostic and research applications. For integrin alpha-1/CD49a-related studies, we provide this -conjugated antibody along with other conjugation options and matched antibody pairs, ensuring flexibility in experimental design. With robust supply capabilities and technical expertise, CamelBio accelerates your development from concept to clinic.