Rabbit anti-Mouse IL1R1/CD121a Monoclonal Antibody for Flow Cytometry - P13504

Core Product Specifications and Parameters

Biological Background: IL1R1/CD121a Function and Localization

• Interleukin-1 receptor type 1 (IL1R1), also known as CD121a, IL-1R alpha, p80, is encoded by the Il1r1 gene in mice.
• Functions as a receptor for interleukin-1 alpha (IL1A), interleukin-1 beta (IL1B), and the IL-1 receptor antagonist (IL1RN), binding these ligands with comparable affinity.
• Upon ligand binding, IL1R1 associates with the coreceptor IL1RAP to form a high-affinity signaling complex, leading to activation of NF-kappa-B and MAPK pathways.
• Signaling involves TIR domain-mediated recruitment of adaptor proteins such as TOLLIP, MYD88, IRAK1, or IRAK2.
• Isoform 2 is expressed in brain tissues and, together with IL1RAP isoform 3, mediates IL1B-induced neuronal activity, including potentiation of NMDA receptor-mediated calcium influx via Akt kinase activation.
• IL1R1 is a transmembrane glycoprotein with disulfide bonds and immunoglobulin-like domains, belonging to the interleukin-1 receptor family.
• Post-translational modifications include phosphorylation and glycosylation; a secreted form has also been identified.
• Plays a role in inflammatory responses and T-helper 1 (Th1) cell costimulation of IFNG production.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 20–338) covers the extracellular domain of mouse IL1R1, making it suitable for detecting surface expression by flow cytometry without permeabilization.
• The conjugate is excited by the 488 nm laser and emits in the green channel (e.g., FITC/GFP); ensure compensation if used in multicolor panels.
• Titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions; typical starting dilutions range from 1:50 to 1:200.
• Include an appropriate isotype control antibody conjugated to the same fluorophore to assess nonspecific binding.
• Mouse Fc receptors can be blocked using anti-CD16/CD32 (Fc Block) to reduce background, especially when using cells with high Fc receptor expression.
• Prepare single-cell suspensions and use a viability dye to exclude dead cells, which may bind antibodies non-specifically.
• Fixation with paraformaldehyde may affect the epitope; validate fixation conditions if post-staining fixation is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive, one-stop access to IVD raw materials and associated technical services. This anti-IL1R1/CD121a antibody exemplifies our portfolio for immunology and inflammation research, where we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. Our flexible supply capabilities help streamline your assay development from concept to clinic, ensuring reliable, high-quality materials tailored to your workflow.