Rabbit anti-Mouse IL-9 mAb - P15247

Core Product Specifications and Parameters

Biological Background: IL-9 Function and Localization

• Interleukin-9 (IL-9), also known as cytokine P40 or T-cell growth factor P40, is a multifunctional cytokine encoded by the Il9 gene in mice.
• It is primarily secreted by T-helper 2 (Th2) lymphocytes, mast cells, and NKT cells, playing a critical role in immune responses against parasites. Related references: PMID:11070175 | PMID:19433802
• IL-9 affects intestinal epithelial permeability and contributes to adaptive immunity, influencing gut homeostasis. Related references: PMID:12704113
• It promotes the differentiation of IL-17-producing helper T-cells (TH17) and stimulates the proliferation and differentiation of mast cells. Related references: PMID:11070175 | PMID:19433802
• IL-9 signals through a heterodimeric receptor composed of the IL-9R alpha chain and the common gamma chain (IL2RG), leading to activation of JAK1/JAK3 kinases and downstream STAT1, STAT3, and STAT5 transcription factors. Related references: PMID:2145361 | PMID:7718508 | PMID:10464327
• The cytokine is secreted and undergoes post-translational modifications, including glycosylation and the formation of pyrrolidone carboxylic acid at the N-terminus.
• IL-9 has a calculated molecular mass of 16075 Da and an observed protein weight of approximately 16 kDa.

Experimental Guidance and Technical Tips

• This antibody targets the mature mouse IL-9 protein (amino acids 19-114), which corresponds to the secreted cytokine after signal peptide cleavage. Consider using it for intracellular flow cytometry following cell stimulation and fixation/permeabilization protocols.
• is a far-red fluorescent dye compatible with common laser lines (e.g., 633-647 nm), making it suitable for multiplex panels. Verify optimal staining concentrations through titration in the intended cell model.
• Ensure proper controls (isotype control, unstimulated cells) to discern specific IL-9 staining, especially given the low expression levels of this cytokine in resting cells.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we serve diagnostic manufacturers, labs, and research institutes by providing comprehensive access to IVD raw materials, technical services, and consulting. This anti-mouse IL-9 monoclonal antibody exemplifies our commitment to offering high-performance immunological reagents for cytokine and immune response studies. From validated antibody pairs and bulk ancillary reagents to rare target sourcing, CamelBio streamlines your path from concept to clinic.