Rabbit anti-Mouse IL-17A Monoclonal Antibody for Flow Cytometry - Q62386

Core Product Specifications and Parameters

Biological Background: Interleukin-17A Function and Localization

• Interleukin-17A (IL-17A), also known as CTLA-8, is a secreted effector cytokine crucial for antimicrobial host defense and maintaining tissue integrity. Related references: PMID:18025225, PMID:19144317, PMID:26431948
• IL-17A signals via the IL17RA-IL17RC heterodimer, recruiting the TRAF3IP2 adapter and activating TRAF6-dependent NF-κB and MAPK pathways to induce inflammatory cytokines, chemokines, and matrix metalloproteinases. Related references: PMID:16200068, PMID:17911633, PMID:19144317
• As the hallmark cytokine of T helper 17 (Th17) cells, IL-17A drives neutrophil recruitment and chemotaxis via CXCL1 and CXCL5 induction in airway epithelium, and supports germinal center formation by regulating B cell responses to CXCL12 and CXCL13. Related references: PMID:18025225, PMID:27923703, PMID:18157131
• Beyond Th17 cells, IL-17A is also produced by innate immune cells including γδ T cells, invariant natural killer T (iNKT) cells, and group 3 innate lymphoid cells (ILC3s), mediating early neutrophilic inflammation and bacterial clearance. Related references: PMID:17372004, PMID:20364087, PMID:28709803, PMID:17470641, PMID:23255360
• IL-17A plays a direct role in epithelial barrier maintenance by regulating tight junction biogenesis, such as occludin (OCLN) localization, following acute injury. Related references: PMID:26431948
• In synergy with IL-17F, IL-17A induces mucosal epithelial cell production of antimicrobial β-defensins (DEFB1, DEFB103A, DEFB104A), restricting microbial entry. Related references: PMID:19144317
• IL-17A contributes to antiviral immunity by enhancing T cell cytotoxicity against West Nile virus and promoting B cell recruitment and differentiation for efficient influenza A virus clearance. Related references: PMID:27795421, PMID:21946434, PMID:26735852
• At the molecular level, IL-17A is a ~18 kDa secreted glycoprotein with a signal peptide, disulfide bonds, and N-linked glycosylation sites.

Experimental Guidance and Technical Tips

• This antibody is pre-conjugated to , enabling direct detection without a secondary antibody; ensure protection from light during handling and storage.
• For intracellular flow cytometry, thorough fixation and permeabilization are required; consider using a commercial intracellular staining kit optimized for cytokines.
• Titrate the antibody to determine the optimal dilution for your specific experimental system and cell type, as staining efficiency can vary.
• Include appropriate controls such as unstimulated cells, isotype control, and fluorescence minus one (FMO) controls when setting up multicolor panels.
• While validated for intracellular flow cytometry (FC intra), performance in other applications (e.g., immunohistochemistry, Western blotting) has not been determined and would require validation in the relevant sample system.
• The immunogen corresponds to amino acids 26–158, covering the mature secreted protein; thus, the antibody likely recognizes the native conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, providing diagnostic manufacturers and research institutions with one-stop access to a comprehensive range of reagents and technical support. For customers developing assays targeting IL-17A in inflammatory, autoimmune, or infectious disease fields, we offer validated antibody pairs, optimized monoclonal antibodies like this one, and bulk ancillary reagents to accelerate your workflow. Our experienced team can also assist in sourcing rare targets and custom raw materials, ensuring a seamless transition from concept to clinic.