Rabbit anti-Mouse IL-13 Monoclonal Antibody for Flow Cytometry (Intracellular) - P20109

Core Product Specifications and Parameters

Biological Background: IL-13 Function and Localization

• Interleukin-13 (IL-13) is a secreted cytokine primarily produced by T-cells, originally identified as T-cell activation protein P600 (UniProt: P20109).
• Plays a key role in allergic inflammation and immune response to parasite infections, synergizing with IL-2 to regulate interferon-gamma synthesis and stimulating B-cell proliferation, eosinophil, basophil, and mast cell activation (By similarity).
• Antagonizes Th1-driven proinflammatory immune responses by downregulating synthesis of proinflammatory cytokines (IL-1, IL-6, IL-10, IL-12, TNF) partially through suppression of NF-kappa-B (By similarity).
• Induces vascular cell adhesion protein 1 (VCAM-1) on endothelial cells, important for eosinophil recruitment, and can affect non-hematopoietic cells. Functions via a receptor complex of IL4R and IL13RA1, activating JAK1, TYK2, and STAT6 (PubMed:34795444, PubMed:8871614). Related references: PMID:34795444, PMID:8871614
• A high-affinity decoy receptor IL13RA2 mediates internalization and depletion of extracellular IL-13 (PubMed:29305434). Related references: PMID:29305434
• Modulates IL33 activity by influencing the production of transmembrane and soluble forms of IL1RL1 (PubMed:34789557). Related references: PMID:34789557
• Induces inflammatory response in esophageal epithelium, leading to dysregulation of desmosome intercellular adhesion junctions via downregulation of DSG1 (PubMed:24220297). Related references: PMID:24220297
• Subcellular location: Secreted. Post-translational modifications include disulfide bonds and glycosylation (predicted molecular weight ~14.1 kDa). (Keywords: Disulfide bond, Glycoprotein, Secreted, Signal).

Experimental Guidance and Technical Tips

• Consider titrating the antibody to determine the optimal working concentration for your specific experimental conditions.
• Perform intracellular staining with appropriate fixation and permeabilization protocols, as the conjugate targets the intracellular cytokine IL-13.
• Use an isotype control to assess non-specific binding and ensure specificity of the staining signal.
• Validate antibody performance in relevant cell models, such as stimulated Th2 cells, mouse splenocytes, or other samples where IL-13 expression is expected.
• For optimal IL-13 detection, stimulation with PMA/ionomycin or other activators may be required to induce cytokine production prior to staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, technical services, and consulting—from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for cytokine and inflammation research. Whether you need anti-IL-13 antibodies or rare target reagents, we provide comprehensive supply solutions to accelerate your diagnostic development.