Rabbit anti-Mouse IL-12 p40 Monoclonal Antibody for Flow Cytometry (Intracellular) - P43432

Core Product Specifications and Parameters

Biological Background: Interleukin-12 subunit beta Function and Localization

• Interleukin-12 subunit beta (IL-12 p40) is a 38.2 kDa secreted glycoprotein that serves as a subunit of two heterodimeric cytokines: IL-12 (p35/p40) and IL-23 (p19/p40).
• As part of IL-12, it acts as a growth factor for activated T and natural killer (NK) cells, enhances the lytic activity of NK/lymphokine-activated killer cells, and stimulates IFN-gamma production by resting peripheral blood mononuclear cells.
• IL-23, formed by the association of IL-12 p40 with IL23A, functions in innate and adaptive immunity, potentially collaborating with IL-17 in acute responses to peripheral tissue infection.
• IL-23 binds to a heterodimeric receptor (IL12RB1/IL23R) and activates the Jak-Stat signaling cascade; it preferentially stimulates memory T cells and promotes the production of pro-inflammatory cytokines.
• IL-23 induces autoimmune inflammation and may contribute to autoimmune inflammatory diseases, as well as play a role in tumorigenesis.
• The protein localizes to the secretory pathway and is released extracellularly; it contains an immunoglobulin domain, disulfide bonds, and glycosylation sites.

Experimental Guidance and Technical Tips

• This antibody is an -conjugated rabbit monoclonal antibody suitable for direct intracellular flow cytometry. Ensure proper fixation and permeabilization protocols for optimal detection of cytoplasmic IL-12 p40.
• The immunogen spans the mature protein (residues 23-335), covering regions involved in receptor binding and heterodimerization; however, performance for detection of native IL-12/IL-23 complexes may require empirical validation in the relevant sample system.
• Due to the key role of IL-12 p40 in immune responses, consider co-staining with other lineage markers to profile cytokine-producing cell subsets.
• exhibits excitation/emission maxima at ~650/665 nm and should be used with appropriate laser/filter configurations; avoid photobleaching by minimizing light exposure.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides end-to-end support for IVD raw material needs, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for immunology and inflammation research. Our IL-12/IL-23 product portfolio supports diagnostic developers in accelerating assay development for cytokine detection and autoimmune disease monitoring. For customized targets or large-scale procurement, our global sourcing network ensures reliable access to rare and research-grade materials.