Rabbit Anti-Mouse IgM Monoclonal Antibody for Flow Cytometry - P01872

Core Product Specifications and Parameters

Biological Background: IgM Heavy Constant Region Function and Localization

• Immunoglobulin heavy constant mu (IgM heavy chain) is encoded by the Ighm gene in mice; it belongs to the immunoglobulin superfamily and represents the first antibody isotype expressed during B cell development.
• It functions as the constant region of both membrane-bound B cell receptors (BCR) and secreted immunoglobulin M (sIgM). The membrane form provides tonic survival signals and mediates pre-BCR signaling, while secreted IgM assembles into pentamers or hexamers for high-avidity antigen binding.
• Secreted IgM (sIgM) mediates humoral effector functions by recruiting complement component C1q to initiate the classical pathway and by binding high-affinity Fc receptor FCMR on lymphoid cells, promoting IgG responses while maintaining self-tolerance. Related references: PMID:10899913 PMID:11062505 PMID:28135254
• IgM participates in mucosal immunity: sIgM is transported across mucosal epithelia by the polymeric immunoglobulin receptor (PIGR) and forms antigen complexes that are retrotranscytosed via FCMR to antigen-presenting cells. Related references: PMID:34788614
• The protein localizes to the cell membrane as part of the BCR and is also secreted; expression is detected in bone marrow and B-1 and B-2 cell subsets.
• Post-translational modifications include N-linked glycosylation and multiple disulfide bonds essential for proper folding and multimerization; alternative splicing generates membrane-bound and secreted isoforms.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the conjugate to determine the optimal dilution for your cell type and fixation/permeabilization protocol.
• Include appropriate isotype control and a viability dye to exclude dead cells from the analysis.
• Consider using an Fc receptor blocking reagent to minimize non-specific binding, especially when working with myeloid cells.
• Validate staining with positive and negative control samples known to express IgM at expected levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, technical services, and consulting. This Rabbit anti-Mouse IgM monoclonal antibody supports flow cytometry-based B cell immunophenotyping and immunology research. We offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your diagnostic development pipeline.