Rabbit anti-Mouse IgM Monoclonal Antibody for Flow Cytometry - P01872

Core Product Specifications and Parameters

Biological Background: Ig Heavy Constant Mu Function and Localization

• Immunoglobulin heavy constant mu (IgM heavy chain constant region) is encoded by the Ighm gene (Igh-6) and constitutes the constant region of IgM antibodies.
• Membrane-bound IgM (mIgM) is a core component of the B cell receptor (BCR) complex, providing constitutive tonic signaling essential for B cell survival and mediating pre-BCR signaling that regulates B cell selection and allelic exclusion.
• Secreted IgM (sIgM) multimerizes into mainly pentamers (occasionally hexamers), conferring high avidity antigen recognition. Natural sIgM are polyreactive, recognizing self and pathogen structures, while immune sIgM are antigen-specific; both are required for an efficient humoral immune response. Related references: PMID:10899913 PMID:28135254 PMID:34788614
• sIgM mediates effector functions primarily through Fc receptors (e.g., FCMR on lymphoid cells) and the complement system. It recruits C1q to initiate the classical complement pathway and, together with C1q and mannose-binding lectin, promotes phagocytosis of apoptotic cells by macrophages, aiding clearance of autoimmunogenic epitopes. Related references: PMID:10899913 PMID:11062505 PMID:28135254
• In mucosal immunity, sIgM is transported across mucosal epithelium via the polymeric immunoglobulin receptor (PIGR) to scan the mucosal lining. IgM-antigen complexes can undergo FCMR-mediated retrotranscytosis across M cells toward antigen-presenting cells in mucosal lymphoid tissues. Related references: PMID:34788614
• Subcellular localization: Secreted form is released into extracellular space; membrane-bound form resides in the cell membrane with a single-pass transmembrane domain.
• Tissue specificity: Expressed in bone marrow and in B-1 and B-2 cells (at protein level).
• Post-translational modifications include disulfide bond formation and glycosylation, critical for proper folding and multimerization.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 105-454 of the mouse IgM constant region; thus, the antibody is expected to recognize the constant domain of all mouse IgM subclasses irrespective of variable region specificity.
• For flow cytometry, titrate the antibody on mouse splenocytes or B cell lines to determine the optimal staining concentration. Consider co-staining with a pan-B cell marker (e.g., anti-CD19) to confirm specificity.
• The fluorophore is a far-red dye (excitation ~650 nm, emission ~668 nm). Ensure your cytometer is equipped with an appropriate red laser (e.g., 633 nm or 640 nm).
• If performing intracellular staining, optimize fixation and permeabilization conditions, as the epitope resides within the constant region and may be preserved after permeabilization.
• Include an isotype control (rabbit monoclonal conjugated to the same fluorophore) to account for non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, laboratories, and research institutes as a one-stop partner for IVD raw materials. For targets like mouse IgM involved in humoral immunity and B cell biology, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing of rare-target raw materials. Partner with us to streamline your assay development from concept to clinic.