Rabbit anti-Mouse IgM Monoclonal Antibody for FC - P01872

Core Product Specifications and Parameters

Biological Background: IgM Function and Localization

• Immunoglobulin heavy constant mu (IgM) is the constant region of immunoglobulin heavy chains, serving as both the B cell receptor (BCR) when membrane-bound and as secreted antibodies (sIgM) that mediate humoral immunity.
• Secreted IgM multimerizes into predominantly pentamers and hexamers, providing multivalent antigen binding and high avidity recognition. Natural sIgM are polyreactive, while immune sIgM are antigen-specific; both are essential for efficient humoral immune responses.
• sIgM effector functions are mediated through Fc receptors (e.g., FCMR) and complement component C1q, promoting induction of neutralizing IgG responses, classical complement pathway activation, and phagocytosis of pathogens and apoptotic cells. Related references: PMID:10899913 PMID:11062505 PMID:28135254
• Membrane-bound IgM (mIgM) constitutes the BCR, providing tonic signaling for B cell survival and mediating pre-BCR signaling that regulates allelic exclusion and B cell development.
• IgM is located at the cell membrane and is also secreted. It is expressed in bone marrow and B-1/B-2 cells at the protein level.
• The protein undergoes alternative splicing to generate secreted and membrane-bound isoforms, and features immunoglobulin domains, glycosylation, and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 105-454 of mouse IgM (P01872), covering the constant region. This design promotes specific recognition of the IgM heavy chain without cross-reactivity to other immunoglobulin isotypes.
• For flow cytometry, titrate the antibody to determine the optimal working concentration for your cell type. Include appropriate isotype controls and validate in mouse B cell lines or primary splenocytes.
• Consider pairing with other B cell markers for detailed immunophenotyping panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete portfolio of IVD raw materials, including validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents, to support diagnostic manufacturers and research labs from concept to clinic. Backed by extensive sourcing expertise, we help accelerate your assay development for B cell biology and humoral immunity applications. Explore our anti-IgM antibodies and custom solutions tailored to your workflow needs.