Rabbit Anti-Mouse IgG2b Monoclonal Antibody for FC - P01867-2

Core Product Specifications and Parameters

Biological Background: Mouse IgG2b Function and Localization

• Mouse IgG2b is an immunoglobulin heavy chain constant region of the gamma 2B isotype. It serves as a structural component of antibody molecules mediating effector functions.
• The protein is encoded by the Ighg2b gene (also known as Igh-3), located within the immunoglobulin heavy chain locus.
• Isoform 2 (UniProt: P01867-2) represents the secreted form of the heavy chain, lacking a transmembrane domain, and is found in serum and extracellular fluids.
• Subcellular localization is exclusively secreted; the mature protein is assembled with light chains and other heavy chains into functional antibodies.
• Alternative names for this protein include Ig gamma-2B chain C region and IgG2b.
• The theoretical molecular weight calculated from the amino acid sequence is approximately 36.6 kDa, while the product observes a band at 37 kDa.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , a green-fluorescent dye compatible with standard flow cytometry lasers and filters.
• The immunogen spans a region within the constant domain; verify sequence homology of the epitope if testing across species or isoforms.
• For flow cytometry, perform antibody titration to determine the optimal staining concentration for your specific cell population and instrument.
• Include appropriate isotype controls and consider Fc receptor blocking when working with mouse samples to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams by providing one-stop access to high-quality IVD raw materials. This anti-mouse IgG2b antibody supports immunophenotyping and isotype quantification applications. Beyond individual reagents, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials to streamline your development pipeline.