Rabbit anti-Mouse IgG1 Monoclonal Antibody for Flow Cytometry - P01868

Core Product Specifications and Parameters

Biological Background: Mouse IgG1 Constant Region Function and Localization

• The Ig gamma-1 chain C region secreted form is the constant region of the mouse IgG1 heavy chain, a major immunoglobulin subclass involved in humoral immunity.
• It is secreted as part of IgG1 antibodies, mediating effector functions such as complement activation and binding to Fc receptors.
• Contains immunoglobulin domains (variable and constant) that form the antigen-binding and effector domains of the antibody.
• Features disulfide bonds that covalently link heavy and light chains, crucial for antibody quaternary structure and stability.
• Is a glycoprotein, with N-linked glycosylation at conserved sites that can influence antibody stability, secretion, and effector functions.
• Alternative splicing generates both secreted and membrane-bound isoforms; the membrane-bound form serves as the B cell receptor for antigen recognition.
• Acts as a reference proteome component, providing a well-characterized target for immunoglobulin research and antibody development.

Experimental Guidance and Technical Tips

• This antibody is a directly conjugated rabbit monoclonal targeting mouse IgG1 constant region (aa 98-324). It is designed for flow cytometry (FC).
• Use an appropriate isotype control antibody conjugated to the same fluorophore to set gates and account for non-specific binding.
• Titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions (e.g., 10^6 cells per test).
• Confirm that the biological samples express secreted or surface IgG1; the antibody will detect IgG1+ B cells or cells coated with secreted IgG1.
• If staining intracellular IgG1, consider fixation and permeabilization protocols; note that the immunogen lies within the constant region and may be accessible under denaturing conditions.
• Validate specificity in the relevant mouse strain; cross-reactivity is reported as mouse-specific, so test on mouse cells and consider potential reactivity with other Ig subclasses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development from concept to clinic. This anti-mouse IgG1 monoclonal antibody facilitates isotype-specific detection in immunological assays, and CamelBio can further assist with custom antibody sourcing, assay buffer optimization, and rare-target raw-material procurement to meet your research and manufacturing needs.