Rabbit anti-Mouse IgG1 Monoclonal Antibody for Flow Cytometry - P01868

Core Product Specifications and Parameters

Biological Background: Ig gamma-1 chain C region secreted form Function and Localization

• The Ighg1 gene encodes the Ig gamma-1 chain C region, which undergoes alternative splicing to produce secreted and membrane-bound forms.
• As a secreted glycoprotein, the Ig gamma-1 chain is a core component of immunoglobulin G1 (IgG1) antibodies, featuring a characteristic immunoglobulin domain structure.
• The protein contains disulfide bonds that stabilize the immunoglobulin fold and facilitate inter-chain linkages.
• Subcellularly, the secreted form is localized to the extracellular space and bodily fluids, consistent with its effector functions in adaptive immunity.
• Post-translational modifications include glycosylation, which can influence protein stability, solubility, and Fc receptor interactions.
• The constant region provides a scaffold for Fc receptor binding and complement activation, mediating antibody-dependent cellular cytotoxicity and pathogen neutralization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 98-324 of the mouse IgG1 constant region, enabling specific detection of the heavy chain in secreted and membrane-bound IgG1.
• For flow cytometry applications, consider titrating the conjugate to optimize signal-to-noise ratio, and include appropriate isotype controls.
• Validate performance in the relevant mouse sample system (e.g., spleen cells, hybridomas) and assess cross-reactivity with other mouse immunoglobulin isotypes if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a one-stop supply of IVD raw materials, including validated monoclonal antibodies, optimized conjugates, and ancillary reagents. This anti-mouse IgG1 antibody supports flow cytometry-based immunophenotyping and mouse model studies. For bulk orders, custom conjugations, or sourcing of rare antibody targets, our team offers tailored solutions to accelerate your assay development from concept to clinic.