Rabbit anti-Mouse IgE Monoclonal Antibody for Flow Cytometry (Intracellular) - P06336

Core Product Specifications and Parameters

Biological Background: Mouse IgE Heavy Chain Constant Region Function and Localization

• Immunoglobulin heavy constant epsilon (IgE heavy chain) is the constant region of immunoglobulin epsilon heavy chains, which together with light chains form IgE antibodies.
• IgE antibodies are glycoproteins produced by B lymphocytes; they can exist as membrane-bound B cell receptors or as secreted antibodies found in serum and mucosal secretions.
• The variable domains of heavy and light chains assemble through V(D)J recombination and undergo somatic hypermutation upon antigen exposure, enabling affinity maturation for specific antigens.
• Membrane-bound IgE on B cells serves as an antigen receptor; antigen binding triggers B cell clonal expansion and differentiation into antibody-secreting plasma cells.
• Secreted IgE mediates effector functions by binding high-affinity Fc epsilon receptors on mast cells and basophils, playing a central role in allergic responses and defense against parasites.
• The IgE constant region contains multiple immunoglobulin domains, and its structure is stabilized by disulfide bonds and N-linked glycosylation (UniProt keywords: Disulfide bond, Glycoprotein, Immunoglobulin domain).
• Subcellularly, IgE is located both on the cell membrane (as a B cell receptor) and secreted into the extracellular space.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a far-red fluorescent dye compatible with standard flow cytometry instruments; it is recommended for intracellular staining (FC intra).
• The immunogen covers amino acids 83–421 of mouse IgE constant region, so the antibody likely recognizes both membrane-bound and secreted IgE forms after permeabilization.
• For intracellular flow cytometry, optimize fixation and permeabilization conditions using appropriate positive and negative control cells (e.g., IgE-expressing mouse B cells vs. IgE-negative lymphocytes).
• Consider incorporating an isotype control and a viability dye in the panel to distinguish specific staining.
• When designing multicolor panels, note that is spectrally similar to APC and Alexa Fluor® 647; avoid co-staining with these dyes unless proper compensation is verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing IVD manufacturers and research laboratories with a comprehensive portfolio of raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This Rabbit anti-Mouse IgE mAb targets the constant region of mouse IgE, supporting immunology and allergy research applications such as B cell phenotyping. Through our one-stop sourcing platform, we also offer tailored procurement of rare-target antibodies and custom reagent scaling to accelerate your diagnostic development from concept to clinic.