Rabbit Anti-Mouse IgE Monoclonal Antibody for Flow Cytometry (Intracellular) - P06336

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Heavy Constant Epsilon Function and Localization

• Immunoglobulin heavy constant epsilon (IGHE), also known as Ig epsilon chain C region, is the constant region of the epsilon heavy chain of immunoglobulin E (IgE).
• IgE antibodies are secreted glycoproteins produced by plasma cells, mediating effector functions in humoral immunity, particularly in allergic responses and defense against parasites.
• Membrane-bound IgE serves as a B-cell receptor (BCR) on B lymphocytes, triggering clonal expansion and differentiation upon antigen binding.
• The antigen-binding site of IgE is formed by the variable domain of the heavy chain paired with a light chain, allowing high-affinity binding to specific antigens after V(D)J recombination and somatic hypermutation.
• IgE is found both secreted in extracellular fluids and as a membrane-bound form on B cells, consistent with its dual roles in immunity.
• The protein undergoes post-translational modifications including disulfide bond formation and glycosylation, and contains immunoglobulin domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 83-421 of the mouse IgE constant region; consider validating reactivity with native and denatured protein forms in your assay.
• For intracellular flow cytometry, optimize fixation and permeabilization protocols to detect intracellular IgE, such as in B cells or mast cells.
• Use this conjugate for direct immunofluorescence staining and include appropriate isotype controls to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, providing validated antibodies and reagents for immunological research and diagnostics. For studying IgE-mediated disorders, we supply high-quality monoclonal antibodies like this anti-mouse IgE (clone ) and can source customized raw materials to support your assay development and manufacturing needs. Trust CamelBio to streamline your supply chain from concept to clinic.